| Field | Specification |
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Product Overview
This product is suitable for the extraction of various viral RNA or DNA from plasma, whole blood, cell-free body fluids (including plasma, serum, urine, CSF and cell culture supernatant), viral stock solution and infected tissues. After the nucleic acid released from the lysed virus binds to the purification column, the column is washed with Buffer WBR to remove residual PCR inhibitors, and the nucleic acid is then eluted with Buffer TE for PCR or RT-PCR.
A 200 µL sample is combined with 20 µL of Protease K storage solution and 200 µL of Buffer REL containing Carrier RNA, vortexed for 15 s and incubated at 56°C for 10 min. After adding 320 µL of ethanol the mixture is loaded onto the nucleic acid purification column at 12,000 rpm for 30 s, washed with 700 µL of Buffer WBR, dried at 14,000 rpm for 1 min and eluted with 50 µL of preheated Buffer TE at 56°C. The extracted nucleic acid is stored at -20°C.
Specifications
| Format | Spin column (nucleic acid purification column) |
|---|---|
| Sample types | Plasma, whole blood, cell-free body fluids, viral stock solution, infected tissues |
| Sample input | 200 µL |
| Nucleic acids recovered | Viral RNA or DNA |
| Chemistry | No organic extraction or ethanol precipitation |
| Special treatment | Carrier RNA supplied |
| Elution volume | 50 µL Buffer TE at 56°C |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Nucleic acid purification column | 50 | 200 |
| Protease K storage solution | 1.1 mL | 4.4 mL |
| Carrier RNA | 400 µL | 1.6 mL |
| Buffer REL | 15 mL | 60 mL |
| Buffer WBR | 60 mL | 240 mL |
| Buffer TE | 5 mL | 20 mL |
Applications
- Viral RNA and DNA extraction from plasma, serum and whole blood
- Nucleic acid extraction from urine, CSF and cell culture supernatant
- Nucleic acid extraction from viral stock solution and infected tissue
- Template preparation for PCR and RT-PCR
Key Features
- Add Carrier RNA for rapid purification to obtain high-quality viral RNA.
- No organic extraction or ethanol precipitation.
- High repeatability and high output.
- Removal of pollutants and inhibitors to facilitate downstream applications.
- The manufacturer states that detection sensitivity can be increased by 10–50 times compared with the traditional boiling method.
- The manufacturer states that detection sensitivity can be increased by 5–10 times compared with the traditional Trizol method.
Storage & Handling
Protease K storage solution and Carrier RNA are stored at -20°C. Other reagents and materials keep their performance unchanged for two years at room temperature (15–25°C); storage at 2–8°C extends the product life beyond two years.
Usage Notes
- Use DNase-free and RNase-free tubes and filter pipette tips.
- Avoid contact between the solution and the edge of the column nozzle.
- Confirm that ethanol has been added to Buffer WBR before use.
- Do not omit the high-speed spin that removes residual ethanol.
- Store the extracted nucleic acid at -20°C.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Molecular characterization of genetically related co-circulating bovine viral diarrhea virus in cattle and water buffalo within mixed-herd production systems. PREVENTIVE VETERINARY MEDICINE, 2026. 10.1016/j.prevetmed.2026.106917
