| Field | Specification |
|---|---|
| Alternative Names | FLJ12930; HB 58; HB58; Hbeta 58; Hbeta 58 homolog; Hbeta58; Hbeta58 homolog; hVPS 26; hVPS26; MGC94168; PEP 8A; PEP8A; Vacuolar protein sorting 26 A; Vacuolar protein sorting 26; Vacuolar protein sorting 26 homolog A; Vacuolar protein sorting 26 homolog A S. pombe; Vacuolar protein sorting 26 homolog A yeast; Vacuolar protein sorting 26 yeast; Vacuolar protein sorting 26 yeast homolog; Vacuolar protein sorting 26A; Vacuolar protein sorting associated protein 26A; Vacuolar protein sorting-associated protein 26A; Vesicle protein sorting 26; Vesicle protein sorting 26A; VP26A_HUMAN; VPS 26; VPS 26A; VPS26A |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Vacuolar protein sorting-associated protein 26A protein (21-180AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-VPS26A antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect VPS26A protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human Vacuolar protein sorting-associated protein 26A protein (21-180AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
VPS26A (also referred to as FLJ12930, HB 58, HB58, Hbeta 58, Hbeta 58 homolog, Hbeta58, Hbeta58 homolog, hVPS 26, hVPS26, MGC94168, PEP 8A, PEP8A, Vacuolar protein sorting 26 A, Vacuolar protein sorting 26, Vacuolar protein sorting 26 homolog A, Vacuolar protein sorting 26 homolog A S. pombe, Vacuolar protein sorting 26 homolog A yeast, Vacuolar protein sorting 26 yeast, Vacuolar protein sorting 26 yeast homolog, Vacuolar protein sorting 26A, Vacuolar protein sorting associated protein 26A, Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26, Vesicle protein sorting 26A, VP26A_HUMAN, VPS 26, VPS 26A, VPS26A) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of VPS26A biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
VPS26A is a protein target in Human biology. This polyclonal antibody raised in Rabbit is designed to detect VPS26A in ELISA, IHC applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, IHC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known VPS26A-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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