| Field | Specification |
|---|---|
| Target | |
| Alternative names | DC15; DC7; DKFZp564F0223; FLJ20492; hVPS29; PEP11; PEP11 homolog; Retromer protein; Vacuolar protein sorting 29; Vacuolar protein sorting 29 (yeast homolog); Vacuolar protein sorting 29 (yeast); Vacuolar protein sorting 29 homolog (S. cerevisiae); Vacuolar protein sorting 29 homolog; Vacuolar protein sorting associated protein 29; Vacuolar protein sorting-associated protein 29; Vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; VPS 29; VPS29; VPS29 retromer complex component; VPS29_HUMAN; x 007 protein |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Vacuolar protein sorting-associated protein 29 protein |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-VPS29 antibody raised in Rabbit, tested in ELISA, WB, IHC. Designed to detect VPS29 in Human, Mouse for signal transduction research.
Key elements and design rationale
- Immunogen: Recombinant Human Vacuolar protein sorting-associated protein 29 protein — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Antigen affinity purification): Enriches Ig classes; reduces non-specific background.
Biological background
VPS29 (also: DC15, DC7, DKFZp564F0223, FLJ20492, hVPS29, PEP11, PEP11 homolog, Retromer protein, Vacuolar protein sorting 29, Vacuolar protein sorting 29 (yeast homolog), Vacuolar protein sorting 29 (yeast), Vacuolar protein sorting 29 homolog (S. cerevisiae), Vacuolar protein sorting 29 homolog, Vacuolar protein sorting associated protein 29, Vacuolar protein sorting-associated protein 29, Vacuolar sorting protein VPS29/PEP11, Vesicle protein sorting 29, VPS 29, VPS29, VPS29 retromer complex component, VPS29_HUMAN, x 007 protein) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current VPS29 annotation relevant to signal transduction.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
- WB: Confirms MW, distinguishes isoforms under denaturing conditions.
- IHC: Tissue expression in FFPE/frozen sections; optimize antigen retrieval.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human, Mouse; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
VPS29 is a protein studied in Human, Mouse biology. This polyclonal antibody raised in Rabbit detects VPS29 in ELISA, WB, IHC applications, IgG isotype.
Reported reactive against Human, Mouse. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA, WB, IHC. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing VPS29; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
