| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | dJ461P17.6 antibody; EDDM4 antibody; epididymal protein 4 antibody; Epididymal secretory protein E4 antibody; Epididymis specific whey acidic protein type four disulfide core antibody; HE 4 antibody; Major epididymis specific protein E4 antibody; Major epididymis-specific protein E4 antibody; MGC57529 antibody; Putative protease inhibitor WAP5 antibody; WAP 5 antibody; WAP domain containing protein HE4 antibody; WAP domain containing protein HE4 V4 antibody; WAP four disulfide core domain 2 antibody; WAP four disulfide core domain protein 2 antibody; WAP four-disulfide core domain protein 2 antibody; WAP5 antibody; WFDC 2 antibody; WFDC2 antibody; WFDC2_HUMAN antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human WFDC2 protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-WFDC2 antibody raised in Human, with confirmed utility in ELISA, IHC. It is designed to detect WFDC2 protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human WFDC2 protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Human): Requires anti-human-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG1): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
WFDC2 (also referred to as dJ461P17.6 antibody, EDDM4 antibody, epididymal protein 4 antibody, Epididymal secretory protein E4 antibody, Epididymis specific whey acidic protein type four disulfide core antibody, HE 4 antibody, Major epididymis specific protein E4 antibody, Major epididymis-specific protein E4 antibody, MGC57529 antibody, Putative protease inhibitor WAP5 antibody, WAP 5 antibody, WAP domain containing protein HE4 antibody, WAP domain containing protein HE4 V4 antibody, WAP four disulfide core domain 2 antibody, WAP four disulfide core domain protein 2 antibody, WAP four-disulfide core domain protein 2 antibody, WAP5 antibody, WFDC 2 antibody, WFDC2 antibody, WFDC2_HUMAN antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of WFDC2 biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG1 from Human) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
WFDC2 is a protein target in Human biology. This monoclonal antibody raised in Human is designed to detect WFDC2 in ELISA, IHC applications, with IgG1 isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, IHC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG1 antibody requires a compatible secondary. Select anti-human IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG1.
(1) Isotype control — IgG1 from Human at matching concentration; (2) Positive control — known WFDC2-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
