| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C31H43N2O18P3 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
WH-15 is a fluorogenic PLC reporter with Km values of 49, 30, and 86.1 μM for PLC-γ1, PLC-δ1, and PLC-β2, respectively. It can be cleaved in a cascade reaction to generate fluorescent 6-aminoquinoline, and it can be used to respond to imaging PLC activity in live cells (Ex/Em = ~344/530 nm)[1]. It is supplied as a light yellow to yellow solid (C31H43N2O18P3, MW 824.60) at 99.51% purity.
Physical & Chemical Properties
| CAS Number | 1264748-47-1 |
|---|---|
| Molecular Formula | C31H43N2O18P3 |
| Molecular Weight | 824.60 g/mol |
| Purity | 99.51% |
| Appearance | Solid |
| Color | Light yellow to yellow |
| SMILES | O[C@@H]1[C@H](OP(O)(OC2=CC=C(COC(NC3=CC=C4N=CC=CC4=C3)=O)C=C2OCCCCCCCC)=O)[C@@H](O)[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@@H]1O |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 25 mg/mL (30.32 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | -20°C, protect from light, stored under nitrogen. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 25 mg/mL (30.32 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light, stored under nitrogen; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
Guide (this recommended protocol is a guideline only and can be adjusted to specific needs)[1]. 1. Solution preparation 1.1 Stock solution preparation Solvent: DMSO. Concentration: 25 mM (optimize per experiment). 1.2 Working solution preparation Dilute to 50 μM in assay buffer. Note: Prepare the working solution and use it immediately, protected from light. 2. Cell staining 2.1 Transfect HEK-293T cells with the indicated DNA (600 ng) and empty vector (100 ng). 2.2 At 37°C, add 70 μL of 50 μM WH-15 (in 2 mM DTT, 3 mM EGTA, 3 mM CaCl2, 70 mM KCl, 50 mM HEPES (pH 7.2) and 83 μg/mL fatty-acid free BSA) to 50 μL of normalized cell lysate. 2.3 Collect data every 3 min using a Multilabel Counter (Ex/Em = 340/535 nm).
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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