| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Whitlow/218 Linker (E3U7Q); Whitlow linker |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Whitlow linker-KLH |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Storage | |
| Target |
Overview
This is a monoclonal anti-Whitlow linker antibody raised in Mouse, conjugated with FITC, with confirmed utility in FC. It is designed to detect Whitlow linker protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Whitlow linker-KLH — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Mouse): Requires anti-mouse-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG2b): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- FITC conjugate: Direct fluorescence (Ex ~490/Em ~525 nm). Protect from light; use antifade reagents for slide-based applications.
Biological background
Whitlow linker (also referred to as Whitlow/218 Linker (E3U7Q), Whitlow linker) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of Whitlow linker biology in others.
Common research applications
- FC: Applicable for FC-based detection; refer to product documentation for optimized conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG2b from Mouse) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a monoclonal antibody produced from mouse hybridoma cells.
Whitlow linker is a protein target in Human biology. This monoclonal antibody raised in Mouse is designed to detect Whitlow linker in FC applications, with IgG2b isotype.
Reported reactive against Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for FC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
FITC direct fluorescence (Ex ~490/Em ~525 nm). Protect from light; use antifade for slide applications.
(1) Isotype control — IgG2b from Mouse at matching concentration; (2) Positive control — known Whitlow linker-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
