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Product Overview
This product can rapidly separate and purify genomic DNA from 200–400 µL of fresh or frozen human or animal whole blood within 15–20 min. The product does not use proteinase K. After the whole blood is dissolved by Solution BFL1, the hemoglobin is removed by Solution BFL2 precipitation. The genomic DNA in the supernatant can be bound to the purification column. After being washed by Buffer RP and Wash Buffer, the remaining proteins on the membrane and the PCR inhibitors are removed, the genomic DNA is eluted by Elution Buffer and can be used in various molecular biology experiments immediately.
In the protocol, 300 µL of Solution BFL1 is added to 400 µL of blood and vortexed for 30 s, followed by 300 µL of Solution BFL2 and a further 30 s vortex. After centrifugation at 12,000 rpm for 2 min the supernatant is loaded onto Adsorption column G, washed with 500 µL of Buffer RP and twice with 600 µL of Wash Buffer, and eluted with 50–100 µL of Elution Buffer after 2–5 min. Purified DNA has an OD260/OD280 of 1.7–1.9.
Specifications
| Format | Silica gel membrane spin column (Adsorption column G) |
|---|---|
| Sample types | Fresh or frozen human or animal whole blood, anticoagulated blood, buffy coat, poultry blood |
| Sample input | 200–400 µL whole blood |
| Processing time | 15–20 min |
| Chemistry | Phenol/chloroform-free |
| Proteinase K | Not used |
| Purity | OD260/OD280 should be 1.7–1.9 |
| Elution volume | 50–100 µL Elution Buffer |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Solution BFL1 | 20 mL | 80 mL |
| Solution BFL2 | 20 mL | 80 mL |
| Buffer RP | 30 mL | 120 mL |
| Wash Buffer | 60 mL | 240 mL |
| Elution Buffer | 10 mL | 40 mL |
| Adsorption column G | 50 set | 200 set |
Applications
- Genomic DNA extraction from whole blood
- DNA extraction from buffy coat and poultry blood
Key Features
- Fast and low toxicity: silica gel membrane adsorption, without phenol or chloroform.
- Wide application: DNA can be directly extracted from samples such as anticoagulant blood, buffy coat and poultry blood.
- High quality: The purified DNA has the characteristics of high concentration, high purity, and good integrity.
Storage & Handling
All components are stored at room temperature for 1 year.
Usage Notes
- Avoid repeated freeze–thaw cycles of blood samples.
- Do not use less than 400 µL of anticoagulated whole blood per preparation.
- Vortex for 30 s after adding Solution BFL1 and again after adding Solution BFL2.
- Add ethanol to the Wash Buffer before use.
- Centrifuge the column for 2 min to dry it before elution.
- A second elution can be carried out to increase yield.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Impact of ABCB1 (rs1045642) polymorphism on antipsychotic-induced extrapyramidal symptoms in Egyptian patients with schizophrenia. Food and Chemical Toxicology, 2026. 10.1016/j.fct.2026.116181
