WPMY-1 cell

SKU:BHC11101290
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Overview
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WPMY-1 cell is a cell line (Male). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Myofibroblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Morphology Myofibroblast
Growth Properties Adherent
Tissue Prostate, stroma
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Catalog no. Size
305083 1 cryovial
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This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305083
Species Human
WPMY-1 is a human prostatic myofibroblast cell line derived from the peripheral zone of the prostate. This cell line was established from the primary culture of prostatic fibroblasts of a 54-year-old Caucasian male patient. Notably, these cells are characterized by their spindle-shaped morphology and expression of smooth muscle actin, reflecting their myofibroblastic phenotype. WPMY-1 cells are an invaluable tool for studying the stromal-epithelial interactions in the prostate, particularly in the context of prostate cancer progression and development. The WPMY-1 cell line has been utilized extensively in research focused on the paracrine and autocrine signaling mechanisms between prostate cancer cells and their microenvironment. These cells are known to secrete a range of cytokines and growth factors that can influence prostate cancer cell growth, invasion, and metastasis. The WPMY-1 line also serves as a robust model to investigate the effects of various pharmacological agents on the behavior of myofibroblasts within the tumor microenvironment. Furthermore, studies using WPMY-1 have contributed significantly to understanding the role of myofibroblasts in the pathophysiology of benign prostatic hyperplasia (BPH) and the fibrotic changes associated with this condition. In addition to their use in cancer and fibrosis studies, WPMY-1 cells have also been employed in research exploring novel therapeutic targets and drug testing, providing insights into the complex interactions within the prostate gland that contribute to disease. This cell line retains several critical aspects of the parental cells' phenotype and function, making it a versatile and valuable resource in prostate disease research.

SKU:BHC11101290

  • Receptors expressed: Androgen receptor, expressed
  • Protein expression: Fibronectin, Smooth Muscle Alpha-Actin, Vimentin
  • Antigen expression: kallikrein 3, KLK3(prostate specific antigen, PSA), Homo sapiens
  • Tumorigenic: No
  • cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
  • supplements: Supplement the medium with 10% FBS
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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