| Field | Specification |
|---|---|
| Target | |
| Alternative names | WRN;RECQ3;RECQL2;RECQL3 |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant fusion protein of human WRN(NP_000544.2). |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes WRN. Polyclonal Antibodies
Validated Applications
| Applications | Western Blot |
|---|---|
| WB | 1:200-1:1000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Recombinant fusion protein of human WRN(NP_000544.2).
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Immunogen | Recombinant fusion protein of human WRN(NP_000544.2). |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 200 kDa |
| Swiss-Prot | Q14191 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for WRN research from Bioworld Technology Inc, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
