| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | WSS1; YHR134WDNA-dependent metalloprotease WSS1; EC 3.4.24.-; DNA damage response protein WSS1; SUMO-dependent isopeptidase WSS1; Weak suppressor of SMT3 protein 1 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Saccharomyces cerevisiae DNA-dependent metalloprotease WSS1 protein (1-269AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-WSS1 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect WSS1 in Saccharomyces cerevisiae for others research.
Key elements and design rationale
- Immunogen: Recombinant Saccharomyces cerevisiae DNA-dependent metalloprotease WSS1 protein (1-269AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
Biological background
WSS1 (also: WSS1, YHR134WDNA-dependent metalloprotease WSS1, EC 3.4.24.-, DNA damage response protein WSS1, SUMO-dependent isopeptidase WSS1, Weak suppressor of SMT3 protein 1) — studied in Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current WSS1 annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Saccharomyces cerevisiae; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
WSS1 is a protein studied in Saccharomyces cerevisiae biology. This polyclonal antibody raised in Rabbit detects WSS1 in ELISA applications, IgG isotype.
Reported reactive against Saccharomyces cerevisiae. Immunogen from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
HRP: direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing WSS1; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
