| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C18H12N2O2 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
XX-650-23 is a potent CREB inhibitor that blocks CREB function by disrupting the CBP-CREB interaction, and it can be used in AML research[1]. It is supplied as an off-white to yellow solid (C18H12N2O2, MW 288.30) at 99.90% purity.
Physical & Chemical Properties
| CAS Number | 117739-40-9 |
|---|---|
| Molecular Formula | C18H12N2O2 |
| Molecular Weight | 288.30 g/mol |
| Purity | 99.90% |
| Appearance | Solid |
| Color | Off-white to yellow |
| SMILES | O=C(C1=C(C=C2C=CC=CC2=C1)O)NC3=CC=C(C=C3)C#N |
| Signaling Pathway | Epigenetics |
| Solubility | In Vitro: DMSO: 100 mg/mL (346.86 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 100 mg/mL (346.86 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | 2.5 mg/mL (8.67 mM); clear solution; requires sonication |
| How to prepare | Gives a clear solution at 2.5 mg/mL. Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
AML cell growth is inhibited by XX-650-23 (870 nM-2.3 μM; 48 hours), with IC50 values of 870 nM (HL-60), 910 nM (KG-1), 2.0 μM (MOLM-13) and 2.3 μM (MV-4-11)[1]. XX-650-23 (5 μM; 6 or 24 hours) lowers H3K27 acetylation specifically, without acting as a general inhibitor of acetyltransferase activity[1]. XX-650-23 (2 μM; 72 hours) triggers apoptosis via the intrinsic apoptosis pathway, with caspase-3 activation and detectable caspase-9 cleavage, and also downregulates Mcl-1 and bcl-2[1].
Western Blot Analysis[1]
| Cell Line | KG-1 cells |
|---|---|
| Concentration | 5 μM |
| Incubation Time | 6 or 24 hours |
| Result | Decreased H3K27 acetylation expression. |
Apoptosis Analysis[1]
| Cell Line | HL-60 cells |
|---|---|
| Concentration | 2 μM |
| Incubation Time | 72 hours |
| Result | Induced apoptosis in AML cells. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
