| Field | Specification |
|---|---|
| Cellosaurus | CVCL_1893 |
| Species | |
| Applications | |
| Classification | |
| Tissue | |
| Disease | Retinoblastoma |
| Age | |
| Sex | |
| Ethnicity | |
| Growth properties | |
| Biosafety level | |
| Catalog no. (Mfr.) | |
| Main SKU |
The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. The donor had a strong maternal family history of retinoblastoma. Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor.
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- Isoenzymes: PGM1, 1, G6PD, B, ES-D, 1, AK-1, 1, GLO-1, 2, Phenotype Frequency Product: 0.1373
- Reverse transcriptase: Negative
- Karyotype: Hypertriploid, with abnormalities including dicentrics, breaks, pulverizations and minutes
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth.
- seedingDensity: 1 x 105 cells/ml
- fluidRenewal: Every 3 days
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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