| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | An E.coli-derived zebrafish Aff4 recombinant protein (amino acids M1-E60) was used as the immunogen for the Zebrafish Aff4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Aff4 Antibody / AF4/FMR2 family member 4 is an antibody targeting Aff4. Host: Rabbit. Polyclonal. Format: Antigen affinity purified. Applications: WB.
Key elements and design rationale
- Target: Aff4.
- Immunogen: An E.coli-derived zebrafish Aff4 recombinant protein (amino acids M1-E60) was used as the immunogen for the Zebrafish A….
- Species reactivity: Zebrafish.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
The AFF4 gene encodes a scaffold protein that functions as a core component of the super elongation complex (SEC), which is involved in transcriptional regulation during embryogenesis. The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. This gene is mapped to chromosome 5q31.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
