| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Alcama recombinant protein (amino acids K54-I509) was used as the immunogen for the Zebrafish Alcama antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Alcama Antibody / Neurolin is a anti-Zebrafish Alcama Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Alcama
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Alcama is prominently expressed in developing neurons, where it mediates axon fasciculation and growth cone navigation, facilitating the formation of organized neuronal tracts. In addition to its role in neurodevelopment, zebrafish Alcama is expressed in vascular endothelial cells and hematopoietic stem and progenitor cells (HSPCs), where it contributes to vasculogenesis, vascular remodeling, and stem cell niche formation.
Due to its conserved structure and multifunctional roles, zebrafish Alcama is a valuable model for studying cell adhesion dynamics, nervous system wiring, hematopoietic development, and angiogenesis. It is also used in research related to immune cell migration, axon pathfinding disorders, and regenerative medicine.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
