| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Bcl2a recombinant protein (amino acids M1-K228) was used as the immunogen for the Zebrafish Bcl2a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Bcl2a Antibody / Bcl-2 is a anti-BCL2A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: BCL2A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Bcl2a acts by inhibiting the mitochondrial apoptotic pathway, primarily through binding and neutralizing pro-apoptotic proteins such as Bax and Bak. This prevents the release of cytochrome c and the subsequent activation of caspases. Zebrafish bcl2a is expressed in various tissues during embryogenesis, including the developing brain, spinal cord, and hematopoietic tissues, where controlled regulation of cell death is essential for proper morphogenesis and tissue homeostasis.
Due to its conserved function with mammalian BCL2, zebrafish Bcl2a is widely used in studies of cell survival signaling, developmental apoptosis, and disease models involving cancer, neurodegeneration, and immune cell regulation. It also serves as a valuable model for investigating the molecular mechanisms of apoptosis and the effects of pro- and anti-apoptotic compounds in vivo.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
