Zebrafish Fli1 Antibody / Friend leukemia integration 1 transcription factor

SKU:BHA17136419
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NSJ Bioreagents
NSJ Bioreagents
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Overview
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Anti-FLI1 antibody from Rabbit, polyclonal (rabbit origin), Rabbit Ig. Recommended for workflows such as Western blot (WB). Reactivity: Zebrafish. Supplied as antigen affinity purified format.
Target FLI1
Host Rabbit
Reactivity Zebrafish
Application WB
Options selector
Catalog no. Formulation Size
RZ1224 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Formulation: 0.5mg/ml if reconstituted with 0.2ml sterile DI water; Size: 100 ug
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: After reconstitution, the Zebrafish Fli1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No RZ1224
Clonality
  • Polyclonal (rabbit origin)
Host Rabbit
Immunogen E. coli-derived zebrafish Fli1 recombinant protein (amino acids M1-H426) was used as the immunogen for the Zebrafish Fli1 antibody.
Isotype
  • Rabbit Ig
Product Type
  • Antibodies
  • Primary Antibodies
Purity Antigen affinity chromatography
Reactivity
  • Zebrafish
Storage After reconstitution, the Zebrafish Fli1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Target FLI1
UniProt # A0A8M9PLW9

Overview

Zebrafish Fli1 Antibody / Friend leukemia integration 1 transcription factor is a anti-FLI1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.

Key elements and design rationale

  • Target: FLI1
  • Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
  • Format: Antigen affinity purified
  • Applications (as listed): WB

Biological background

Friend leukemia integration 1 transcription factor (FLI1),also known as transcription factor ERGB,is a protein that in humans is encoded by the FLI1 gene,which is a proto-oncogene. It is mapped to 11q24.3. This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22,which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Research relevance and current trends

  • Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
  • Considering isoforms and post-translational regulation when interpreting protein-level changes.
  • Comparing results across species and model systems with matched controls.

Common research applications

  • Western blotting: compare relative abundance and activation-state changes across conditions.

Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.

Notes for experimental interpretation

  • Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
  • Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.

Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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