| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | This LC3B antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 16-50 amino acids from the N-terminal region of zebrafish Map1lc3b. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Zebrafish Map1lc3b is initially synthesized as a cytosolic precursor (LC3-I), which undergoes post-translational cleavage and lipidation to form LC3-II, the autophagosome-associated form. This lipidated form binds to autophagic membranes, making Map1lc3b essential for autophagosome formation, maturation, and cargo recruitment.
In zebrafish, map1lc3b is expressed during early embryogenesis and throughout development, with increased expression observed in tissues undergoing high autophagic flux. It has become a widely used marker in transgenic zebrafish lines (e.g., Tg(CMV:map1lc3b-GFP)) for live imaging of autophagy under physiological and stress conditions, including nutrient deprivation, neurodegeneration, and toxin exposure.
The conserved role of Map1lc3b in autophagy makes it an important tool for studying cellular homeostasis, developmental processes, and disease models in zebrafish.
- Format: Antigen affinity purified
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
