| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Lft1 recombinant protein (amino acids R19-K312) was used as the immunogen for the Zebrafish Lft1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Lft1 Antibody / Lefty | Left-right determination factor is a anti-LFT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: LFT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish embryos, Lft1 is expressed asymmetrically, typically on the left side of the lateral plate mesoderm and near the embryonic midline. Its main role is to inhibit nodal signals from spreading to the right side of the embryo, thereby ensuring that organs such as the heart, liver, and pancreas develop in their correct anatomical positions. Lft1 acts as a feedback inhibitor, maintaining a precise boundary of nodal activity and contributing to the robustness of left right patterning.
Disruption of Lft1 expression or function can result in abnormal or randomized organ laterality, making it a critical component of the left right determination network. Zebrafish Lft1 is also involved in regulating mesoderm formation and is influenced by other early patterning cues such as those from the organizer region.
Due to its conserved role across vertebrates, zebrafish Lft1 is widely used in studies of embryonic asymmetry, developmental signaling, congenital heart defects, and the genetic regulation of organ positioning.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
