| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Luteinizing hormone beta recombinant protein (amino acids L24-Y140) was used as the immunogen for the Zebrafish Luteinizing hormone beta antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Luteinizing hormone beta Antibody / Lhb is a anti-Zebrafish Luteinizing hormone beta Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Luteinizing hormone beta
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
Zebrafish Lhb is produced by gonadotrope cells in the anterior pituitary and is essential for triggering final oocyte maturation and ovulation in females, as well as steroidogenesis and spermatogenesis in males. Its expression is tightly regulated by gonadotropin-releasing hormone (GnRH) and modulated by sex steroids through feedback mechanisms.
The zebrafish model has been instrumental in dissecting the molecular and developmental regulation of lhb expression. Notably, Lhb mRNA becomes detectable during larval stages and increases during sexual maturation. Functional studies using gene knockout and reporter lines have demonstrated that Lhb is indispensable for fertility, and its disruption results in reproductive failure.
Due to its conserved structure and function, zebrafish Lhb serves as a valuable model for studying reproductive endocrinology, pituitary cell differentiation, and hormone signaling pathways in vertebrates.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
