| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish N-cadherin recombinant protein (amino acids M25-R810) was used as the immunogen for the Zebrafish N-cadherin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish N-cadherin Antibody / Ncad / Cdh2 / Cadherin 2 is a anti-CDH2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: CDH2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish N cadherin is the ortholog of the human CDH2 gene, which encodes the same protein. Both zebrafish and human N cadherin are members of the classical cadherin family and share a conserved structure that includes extracellular cadherin repeats, a single transmembrane domain, and a cytoplasmic region that interacts with catenins to link cell adhesion to the actin cytoskeleton.
In zebrafish embryos, N cadherin is expressed in the developing nervous system, heart, somites, and other tissues undergoing epithelial to mesenchymal transitions. It mediates homophilic cell-cell adhesion, ensuring coordinated cell movements and stable tissue organization. Loss of N cadherin function in zebrafish results in severe developmental defects, including impaired brain morphogenesis, defective heart formation, and abnormal cell migration.
Due to its strong evolutionary conservation and fundamental role in cell adhesion and signaling, zebrafish N cadherin is widely used as a model to study developmental biology, tissue engineering, cancer metastasis, and congenital disorders involving cadherin dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
