| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Ndr1 recombinant protein (amino acids Q28-D296) was used as the immunogen for the Zebrafish Ndr1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Ndr1 Antibody / Nodal-related 1 is a anti-NDR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: NDR1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
Zebrafish Nodal related 1 is considered a functional homolog of human NODAL, although it is not a direct one to one ortholog due to lineage specific gene duplications in teleost fish. However, both proteins are part of the same signaling pathway and perform highly conserved roles in vertebrate embryogenesis. Like human NODAL, zebrafish Nodal related 1 signals through the SMAD family of transcription factors and is regulated by feedback mechanisms involving antagonists such as Lefty and Cerberus.
Expression of Nodal related 1 begins during the blastula stage and becomes concentrated in the embryonic margin, where it helps induce and pattern the mesendoderm. Mutations or knockdown of this gene result in severe defects in gastrulation, germ layer formation, and organ development. Due to its essential role in vertebrate development and strong functional conservation, zebrafish Nodal related 1 is widely studied in developmental biology, cell signaling, and disease modeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
