| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Npas4l recombinant protein (amino acids Q94-I647) was used as the immunogen for the Zebrafish Npas4l antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Npas4l Antibody / Cloche / Neuronal PAS domain-containing protein 4-like is a anti-NPAS4L Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: NPAS4L
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Npas4l is unique to zebrafish and closely related teleost species, and it does not have a confirmed one to one ortholog in humans. However, it is functionally analogous to members of the NPAS family of transcription factors in mammals, particularly those involved in neurogenesis and vascular development. Its closest functional equivalents in mammals are believed to include NPAS4 and potentially other early endothelial regulators, although no direct human counterpart fully recapitulates its role in vascular progenitor specification.
In zebrafish embryos, Npas4l is expressed in the lateral plate mesoderm where it acts upstream of critical genes such as etsrp and tal1, which drive endothelial and hematopoietic differentiation. Loss of Npas4l results in the absence of blood and most vascular structures, making it essential for cardiovascular development.
Because of its pivotal and early role in blood and vessel formation, zebrafish Npas4l is a widely used genetic marker and model in studies of hematopoiesis, angiogenesis, and transcriptional regulation. It is especially valuable for dissecting the gene regulatory networks that control endothelial cell fate decisions in vertebrate embryos.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
