| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Ostf1 recombinant protein (amino acids R57-K190) was used as the immunogen for the Zebrafish Ostf1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Ostf1 Antibody / Osteoclast-stimulating factor 1 is a anti-OSTF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: OSTF1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Ostf1 is strongly expressed in the gills, kidney, and other osmoregulatory organs, where it contributes to the adaptation of cells to changes in external ionic and osmotic conditions. Zebrafish Ostf1 is an ortholog of the human OSTF1 gene. Both zebrafish and human proteins share conserved structural features, including domains involved in transcriptional regulation and protein interactions.
Functionally, Ostf1 acts as an intracellular signaling mediator that is activated in response to hyperosmotic stress. In zebrafish, it has been shown to regulate the expression of ion transporters and channels, including those involved in sodium and chloride balance. It may also contribute to the remodeling of gill epithelium and the function of mitochondrion-rich cells during osmotic adaptation.
Ostf1 is also implicated in developmental processes and stress signaling pathways beyond osmoregulation. Because of its conservation and functional importance, zebrafish Ostf1 is widely used in studies of environmental stress physiology, aquaculture research, and basic cell signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
