| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pafah1b1 recombinant protein (amino acids I95-R410) was used as the immunogen for the Zebrafish Pafah1b1 antibody. This antibody will detect the a and b isoforms. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pafah1b1 Antibody / Pafah1b1a / Pafah1b1b / Lissencephaly-1 is a anti-PAFAH1B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PAFAH1B1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Pafah1b1 is an ortholog of the human PAFAH1B1 gene. Both zebrafish and human Pafah1b1 share a high degree of sequence conservation and functional similarity. This conservation makes zebrafish an excellent model for studying the biological functions of Pafah1b1 and its role in various cellular processes. Zebrafish Pafah1b1 is involved in the regulation of inflammatory responses, neurogenesis, and vascular development.
Pafah1b1 is primarily expressed in the brain, heart, and blood vessels during zebrafish development. It plays an essential role in the formation and maintenance of the vascular system, where it helps regulate vascular permeability and inflammation. In addition to its role in vascular biology, Pafah1b1 is important for neuronal development, particularly in neurogenesis and synaptic plasticity.
The zebrafish model is widely used to study developmental processes such as angiogenesis and neurogenesis, and the study of Pafah1b1 in zebrafish offers valuable insights into its functional roles in these pathways. Its involvement in immune modulation and neurodevelopmental diseases also makes zebrafish Pafah1b1 an important protein for modeling human conditions related to vascular diseases, neurological disorders, and inflammatory responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
