| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pax6 recombinant protein (amino acids M1-M401) was used as the immunogen for the Zebrafish Pax6 antibody. This antibody will detect isoforms a & b. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pax6 Antibody / Pax6a / Pax6b is a anti-PAX6 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.
Key elements and design rationale
- Target: PAX6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IF
Biological background
During embryogenesis, zebrafish Pax6 is expressed in the forebrain, optic vesicles, retina, and spinal cord, where it regulates the expression of genes critical for cell fate determination and tissue patterning. Pax6 is a key regulator of eye field specification and is required for the proper formation of structures such as the lens, retina, and optic tectum. In addition, it contributes to pancreatic islet cell development, particularly in the differentiation of insulin-producing Beta-cells.
Given its evolutionary conservation and well-characterized functions, zebrafish Pax6 is widely used as a model for studying ocular development, neural differentiation, congenital eye disorders (e.g., aniridia), and transcriptional control of organogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
