| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pdcd10 recombinant protein (amino acids M1-A210) was used as the immunogen for the Zebrafish Pdcd10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pdcd10 Antibody / Pdcd10a / Pdcd10b / Programmed cell death protein 10 is a anti-PDCD10 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: PDCD10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Pdcd10 is an ortholog of the human PDCD10 gene. Both zebrafish and human Pdcd10 share high sequence similarity and functional conservation, especially in regulating endothelial cell behavior and vascular development. The zebrafish model is particularly useful for studying the role of Pdcd10 in vascular biology, developmental angiogenesis, and its involvement in vascular diseases.
Pdcd10 is involved in the regulation of endothelial cell junctions and vascular permeability. It plays a significant role in vascular remodeling during embryogenesis and in the maintenance of blood-brain barrier integrity. By regulating cell-cell adhesion and controlling the actin cytoskeleton, Pdcd10 ensures proper endothelial cell alignment and the formation of functional blood vessels. It is also involved in cell survival pathways, particularly in protecting endothelial cells from stress-induced apoptosis.
In zebrafish, Pdcd10 is expressed in tissues undergoing active vascularization, such as the brain, heart, and fin. It is particularly important in the developing blood vessels, where it regulates the formation of vascular networks and ensures the proper development of the circulatory system. Pdcd10 is essential for maintaining the balance between angiogenesis and vascular stability during both development and tissue regeneration.
Given its critical role in vascular development, angiogenesis, and endothelial cell survival, zebrafish Pdcd10 is an important model for studying vascular diseases, including cerebral cavernous malformations, stroke, and tumor angiogenesis. Its involvement in vascular integrity and cell survival also makes it a relevant target for research into cardiovascular diseases and vascular dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
