| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Sf3a1 recombinant protein (amino acids K16-P646) was used as the immunogen for the Zebrafish Sf3a1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Sf3a1 Antibody / Splicing factor 3A subunit 1 is a anti-SF3A1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P, Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.
Key elements and design rationale
- Target: SF3A1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P, IF
Biological background
Zebrafish Sf3a1 is an ortholog of the human SF3A1 protein and shares significant sequence and functional conservation. Like its human counterpart, zebrafish Sf3a1 is required for normal RNA processing and proper regulation of gene expression. Due to the importance of splicing in controlling developmental and cellular processes, Sf3a1 function is essential during embryogenesis and tissue differentiation.
Currently, there are no known isoforms of zebrafish Sf3a1 that have been characterized, but as with other splicing factors, alternative splicing may generate additional transcript variants that are yet to be fully studied. Research into zebrafish Sf3a1 offers valuable insights into conserved mechanisms of splicing regulation and its roles in development and disease.
Antibodies targeting zebrafish Sf3a1 protein are useful tools for examining spliceosomal assembly, RNA processing, and transcriptional regulation. These antibodies are commonly used in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, supporting a broad range of molecular and developmental biology studies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
