| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Tbx5a recombinant protein (amino acids M1-S492) was used as the immunogen for the Zebrafish Tbx5a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Tbx5a Antibody / Tbx5 / T-box protein 5a is a anti-TBX5A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: TBX5A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Tbx5a is an ortholog of the human TBX5 protein, sharing significant sequence conservation and functional similarity. In both zebrafish and humans, Tbx5a regulates the formation of the heart's left ventricle and is involved in the proper patterning of the limbs during early development. Mutations in TBX5 in humans are associated with Holt-Oram syndrome, a disorder that leads to congenital heart defects and upper limb abnormalities. The conserved function of zebrafish Tbx5a makes it an ideal model for studying cardiac and limb development and for understanding the underlying genetic causes of congenital malformations.
The use of a Zebrafish Tbx5a antibody allows researchers to monitor the expression of Tbx5a in various developmental stages, particularly in the heart and limb tissues. These antibodies are invaluable for applications such as western blot, immunohistochemistry, and immunofluorescence, enabling detailed study of Tbx5a expression and its role in patterning and organogenesis. A Zebrafish Tbx5a antibody is also useful for assessing the effects of genetic mutations or environmental factors on heart and limb development.
Currently, no distinct isoforms of zebrafish Tbx5a have been identified, but its critical role in cardiac and limb development has been well documented. Using a high-quality Zebrafish Tbx5a antibody, researchers can explore the developmental pathways regulated by Tbx5a and gain insights into congenital diseases related to this transcription factor.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
