| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Tnfa recombinant protein (amino acids M1-K226) was used as the immunogen for the Zebrafish Tnfa antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Tnfa Antibody / Tnf alpha is a anti-Zebrafish Tnfa Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Tnfa
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Tnfa is an ortholog of the human TNF alpha protein and shares structural and functional conservation with its human counterpart. Similar to human TNF alpha, zebrafish Tnfa is synthesized as a membrane bound precursor that can be cleaved into a soluble form, which binds to TNF receptors to initiate signaling. The conserved nature of Tnfa makes zebrafish a valuable model for studying the role of TNF alpha in inflammatory and immune related diseases.
There are no confirmed isoforms of zebrafish Tnfa, and its function appears to be largely analogous to the human protein. Research using zebrafish Tnfa provides insights into cytokine signaling, inflammation, immune cell development, and host pathogen interactions.
Antibodies against zebrafish Tnfa protein are useful for applications such as western blot, immunohistochemistry, immunofluorescence, ELISA, and flow cytometry. These tools help researchers study the dynamics of inflammatory signaling and the effects of therapeutic interventions targeting the TNF alpha pathway.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
