ZNF331 Antibody, HRP conjugated

Overview

This polyclonal anti-ZNF331 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect ZNF331 in Human for epigenetics and nuclear signaling research.

Key elements and design rationale

• Immunogen: Recombinant Human Zinc finger protein 331 protein (179-442AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.

Biological background

ZNF331 (also: C2H2 like zinc finger protein, C2H2 like zinc finger protein rearranged in thyroid adenomas, C2H2-like zinc finger protein rearranged in thyroid adenomas, DKFZp686L0787, KRAB zinc finger protein, OTTHUMP00000223038, OTTHUMP00000223039, OTTHUMP00000223040, OTTHUMP00000223041, OTTHUMP00000223042, OTTHUMP00000223043, OTTHUMP00000223045, OTTHUMP00000223046, OTTHUMP00000223047, OTTHUMP00000223048, OTTHUMP00000223052, OTTHUMP00000223053, OTTHUMP00000223054, RITA, Zinc finger protein 331, Zinc finger protein 361, Zinc finger protein 463, ZN331_HUMAN, ZNF331, ZNF361, ZNF463) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ZNF331 annotation relevant to epigenetics and nuclear signaling.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12555107)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.