96 Well Glass Bottom Plates
Cellvis 96-well plates with a black polystyrene frame and lid, offered with #0 or #1 cover glass, #1.5 high performance cover glass, a tissue culture treated #1.5 glass-like polymer coverslip, or as a low base plate with #1.5 high performance cover glass. Gamma sterilized, conforming to ANSI/SBS 1-2004 and intended for high resolution imaging, for example confocal microscopy.
| Catalog No. | Coverslip | Plate Base | Packaging | Availability | Price | Qty | Order |
|---|---|---|---|---|---|---|---|
| P96-0-N | #0 cover glass (0.085-0.115 mm) | Regular | 20/case | In Stock at Manufacturer | $379.00 |
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| P96-1-N | #1 cover glass (0.13-0.16 mm) | Regular | 20/case | In Stock at Manufacturer | $379.00 |
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| P96-1.5H-N | #1.5H cover glass (0.170 ± 0.005 mm) | Regular | 20/case | In Stock at Manufacturer | $416.00 |
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| P96-1.5P | #1.5 glass-like polymer (0.175 ± 0.010 mm) | Regular | 20/case | In Stock at Manufacturer | $370.00 |
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| P96LB-1.5H | #1.5H cover glass (0.170 ± 0.005 mm) | Low base | 20/case | In Stock at Manufacturer | $416.00 |
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Choose Coverslip, then Plate Base.
Overview
What is 96 Well Glass Bottom Plates?
Product Overview
The Cellvis 96-well plate has a black polystyrene frame with a lid and is intended for high resolution imaging, for example confocal microscopy. It is offered with #0 cover glass (0.085-0.115 mm), #1 cover glass (0.13-0.16 mm), #1.5 high performance cover glass (0.170 ± 0.005 mm) or a tissue culture treated #1.5 glass-like polymer coverslip (0.175 ± 0.010 mm).
A low base format with #1.5 high performance cover glass (P96LB-1.5H) is also available; its bottom height, from the bottom of the coverslip to the plate bottom, is 0.26 mm. Cellvis recommends the polymer coverslip format (P96-1.5P) when better cell attachment is needed.
Key Features
- Suitable for long-term tissue culture
- Manufactured in a class 100,000 clean room
- Frame made from virgin polystyrene
- Glass formats: high optical quality German cover glass; the #1.5 high performance cover glass is 0.170 ± 0.005 mm thick
- P96-1.5P: tissue culture treated #1.5 glass-like polymer coverslip (0.175 ± 0.010 mm) for better cell attachment
- Coverslip bonded to the plate with a USP class VI adhesive
- Sterilized by gamma radiation
- Conforms to ANSI/SBS 1-2004 standards
- Black frame, with lid
Imaging Compatibility
Cellvis lists the cover glass formats (P96-0-N, P96-1-N, P96-1.5H-N, P96LB-1.5H) as suitable for all of the techniques below. The polymer coverslip format (P96-1.5P) is listed for the same techniques except Total Internal Reflection Fluorescence (TIRF), for which it is not suitable.
- Differential Interference Contrast (DIC)
- Widefield Fluorescence
- Confocal Microscopy
- Two-Photon and Multiphoton Microscopy
- Fluorescence Recovery After Photobleaching (FRAP)
- Förster Resonance Energy Transfer (FRET)
- Fluorescence Lifetime Imaging Microscopy (FLIM)
- Total Internal Reflection Fluorescence (TIRF)
- Super-Resolution Microscopy
Cellvis recommends P96-1.5H-N and P96LB-1.5H for confocal microscopy and super-resolution microscopy, and P96-1.5P for confocal microscopy.
Available Formats
| Catalog No. | Coverslip | Plate Base | Pack Size |
|---|---|---|---|
| P96-0-N | #0 cover glass (0.085-0.115 mm) | Regular | 20/case, individually packed |
| P96-1-N | #1 cover glass (0.13-0.16 mm) | Regular | 20/case, individually packed |
| P96-1.5H-N | #1.5H cover glass (0.170 ± 0.005 mm) | Regular | 20/case, individually packed |
| P96-1.5P | #1.5 glass-like polymer (0.175 ± 0.010 mm), tissue culture treated | Regular | 20/case |
| P96LB-1.5H | #1.5H cover glass (0.170 ± 0.005 mm) | Low base | 20/case, individually packed |
Specifications
| Wells | 96 |
|---|---|
| Frame Color | Black |
| Coverslip | #0 (0.085-0.115 mm) or #1 (0.13-0.16 mm) cover glass, #1.5 high performance cover glass (0.170 ± 0.005 mm) or #1.5 glass-like polymer (0.175 ± 0.010 mm) |
| Length | 127.60 mm |
| Width | 85.75 mm; 85.60 mm (P96LB-1.5H) |
| Height | 14.30 mm; 14.40 mm (P96LB-1.5H) |
| Height with Lid | 16.5 mm; 17.64 mm (P96LB-1.5H) |
| Stacking Height (with Lid) | 15.5 mm; 16.70 mm (P96LB-1.5H) |
| Bottom Height | 1.8 mm (P96-0-N); 1.75 mm (P96-1-N); 1.73 mm (P96-1.5H-N, P96-1.5P); 0.26 mm (P96LB-1.5H); bottom of coverslip to plate bottom |
| Bottom Height Tolerance | ± 50 μm (whole plate) |
| Lid Dimension | 127 x 85 x 10 mm; 127 x 85 x 9.3 mm (P96LB-1.5H) |
| Well to Well Center Distance | 9.00 mm |
| Well Bottom Area | 30 mm² (P96-0-N, P96-1-N, P96-1.5H-N, P96-1.5P) |
| Maximum Volume | 0.35 ml (P96-0-N, P96-1-N, P96-1.5H-N, P96-1.5P) |
| Temperature Range | -20°C to 50°C (P96-0-N, P96-1-N, P96-1.5H-N, P96-1.5P) |
| Frame Material | Virgin polystyrene |
| Sterilization | Gamma radiation |
| Plate Standard | ANSI/SBS 1-2004 |
| Pack Size | 20/case |
Source: Cellvis published specifications.
Details
Specifications
Applications
Applications & how to use
Confocal Microscopy
Super-Resolution Microscopy
Differential Interference Contrast (DIC)
Widefield Fluorescence
Two-Photon and Multiphoton Microscopy
Fluorescence Recovery After Photobleaching (FRAP)
Förster Resonance Energy Transfer (FRET)
Fluorescence Lifetime Imaging Microscopy (FLIM)
Total Internal Reflection Fluorescence (TIRF)
Cell Culture (Growth)
Routine expansion and maintenance of cell lines in adherent or suspension format. Reagent quality directly affects doubling time, morphology and the reproducibility of any downstream assay.
Evidence
Validation & QC
Validation data for this product isn’t published yet.
Need specific QC or validation results — titer, purity, endotoxin, or assay data — for your application? Our scientific team can share what is available for this product and lot on request.
Request validation dataManufacturing
How it's made & quality control
Supplier quality control
Manufactured in a class 100,000 clean room.
A USP class VI adhesive is used to bond the coverslip on glass bottom products.
Most products are sterilized by gamma radiation; each product page lists the coverslip type, thickness and dimensions.
Supplier-level quality statement. For lot-specific results, request the Certificate of Analysis for your batch.
Questions
Frequently asked questions
What is the difference between the five formats?
P96-0-N, P96-1-N and P96-1.5H-N have #0, #1 and #1.5 high performance cover glass. P96-1.5P has a tissue culture treated #1.5 glass-like polymer coverslip. P96LB-1.5H is a low base plate with #1.5 high performance cover glass.
What is different about the low base plate?
On P96LB-1.5H the bottom height, measured from the bottom of the coverslip to the plate bottom, is 0.26 mm, compared with 1.73 mm on P96-1.5H-N. Its outer dimensions also differ slightly: 85.60 mm wide, 14.40 mm high and 17.64 mm high with the lid.
When should the polymer coverslip format be chosen?
Cellvis recommends P96-1.5P when better cell attachment is needed. It is not suitable for Total Internal Reflection Fluorescence (TIRF).
What is the well bottom area and maximum volume?
For P96-0-N, P96-1-N, P96-1.5H-N and P96-1.5P the well bottom area is 30 mm² and the maximum volume is 0.35 ml. Cellvis does not list these values for P96LB-1.5H.
How many plates are in a case?
20 per case.
Answered by our team
Questions from researchers
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