| Field | Specification |
|---|---|
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NF-kappaB p65 around the acetylation site of Lys310. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Acetyl-NF-kappaB p65 (Lys310) Antibody detects endogenous levels of Acetyl-NF-kappaB p65 only when acetylated at Lys310. This is a polyclonal Rabbit anti-Acetyl-NF-kappaB p65 antibody, supplied as unconjugated, validated for IF/ICC | IHC | WB.
Validated Applications & Dilution Guide
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:50-1:200 |
| IF/ICC | 1:200 |
Antibody Specifications
| Target Protein | Acetyl-NF-kappaB p65 |
|---|---|
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Purification Method | Affinity Purified (Peptide) |
| Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl. |
| Catalog No. | AF1017 |
Immunogen / Antigen
A synthesized peptide derived from human NF-kappaB p65 around the acetylation site of Lys310.
Predicted cross-reactivity: Pig,Bovine,Horse,Sheep,Dog. Predictions are based on sequence homology and have not been empirically validated unless stated otherwise.
Validation & Citations
✓ Affinity Purified ✓ 12 Citations
RRID: AB_2835385 — registered in the Antibody Registry for unambiguous reagent identification across publications.
This antibody has been independently cited in 12 peer-reviewed publications. Verify records via the Antibody Registry (AB_2835385) or PubMed.
Storage & Handling
Store at -20 °C. Stable for 12 months from date of receipt.
This antibody has been validated for IF/ICC, IHC, WB. Recommended dilutions: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200. Always titrate for your specific cell/tissue type, fixation method, and detection system.
Starting dilutions: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200. Run a 2-fold dilution series (3–4 concentrations) using positive-control samples to identify the optimal working dilution for your assay. Detection chemistry, antigen abundance, and sample preparation all influence optimal antibody concentration.
Confirmed reactivity: Human,Mouse,Rat. Additional predicted species may be listed in the datasheet based on sequence homology analysis. Always empirically validate in your specific organism and tissue type before a full experimental commitment.
A synthesized peptide derived from human NF-kappaB p65 around the acetylation site of Lys310. This polyclonal Rabbit antibody was generated by peptide immunisation and subsequently affinity-purified. The immunogen sequence defines the recognised epitope and governs cross-species reactivity patterns.
Store at -20 °C. Stable for 12 months from date of receipt. Aliquot upon receipt into single-use volumes to prevent repeated freeze-thaw cycles, which progressively degrade antibody performance. Store working dilutions at 4 °C for short-term use only (≤1 week). Do not dilute the stock solution before long-term storage.
This antibody is validated under denaturing conditions standard for WB (SDS-PAGE). For non-denaturing applications (IP, Co-IP), native epitope accessibility may differ — optimize conditions and include appropriate IgG isotype controls. For IHC and IF/ICC, test antigen retrieval conditions empirically as fixation can mask the target epitope.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gene-Activating Framework Nucleic Acid-Targeted Upregulating Sirtuin-1 to Modulate Osteoimmune Microenvironment for Diabetic Osteoporosis Therapeutics. ACS nano (2024) [IF=15.8] PMID: 39689347
Targeting high glucose-induced epigenetic modifications at cardiac level: the role of SGLT2 and SGLT2 inhibitors. Cardiovascular Diabetology (2023) [IF=9.3] PMID: 36732760
Polygonatum sibiricum polysaccharides protect against knee osteoarthritis by inhibiting the TLR2/NF-κB signaling pathway in vivo and in vitro. International journal of biological macromolecules (2024) [IF=7.7] PMID: 38901508
Acetylbritannilactone Modulates MicroRNA-155-Mediated Inflammatory Response in Ischemic Cerebral Tissues. MOLECULAR MEDICINE (2015) [IF=6.0] PMID: 25811992
Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION (2022) [IF=5.9] PMID: 35411569
Baicalin suppresses macrophage JNK-mediated adipose tissue inflammation to mitigate insulin resistance in obesity. Journal of ethnopharmacology (2024) [IF=4.8] PMID: 38762213
Renshen Shouwu formula alleviates Alzheimer's disease pathology by modulating tryptophan metabolism and activating the SIRT1 signaling pathway. Journal of ethnopharmacology (2026) [IF=4.8] PMID: 41423157
Neutrophil extracellular traps in rheumatoid arthritis: Activating fibroblast-like synoviocytes via ATP citrate lyase. International immunopharmacology (2024) [IF=4.8] PMID: 39522314
Resveratrol ameliorates sevoflurane-induced cognitive impairment by activating the SIRT1/NF-κB pathway in neonatal mice. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2021) [IF=4.8] PMID: 33388350
α-Mangostin Treats Early-Stage Adjuvant-Induced Arthritis of Rat by Regulating the CAP-SIRT1 Pathway in Macrophages. Drug Design, Development and Therapy (2022) [IF=4.7] PMID: 35250263
