| Field | Specification |
|---|---|
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Full-length Actin protein of human. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein. This is a monoclonal Mouse anti-actin antibody (Clone AFB18379), supplied as unconjugated, validated for ELISA | IF/ICC | IHC | WB.
Validated Applications & Dilution Guide
| Application | Recommended Dilution |
|---|---|
| WB | 1:3000-1:10000 |
| IHC | 1:50-1:200 IF/ICC: 1:200 |
Antibody Specifications
| Target Protein | actin |
|---|---|
| Host Species | Mouse |
| Clonality | Monoclonal (Clone AFB18379) |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat, Pig, Zebrafish, Bovine, Sheep, Rabbit, Goat, Guinea pig, Dog, Monkey, Hamster, Chicken, Fish |
| Purification Method | Affinity Purified |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Catalog No. | T0021 |
Immunogen / Antigen
Full-length Actin protein of human.
Validation & Citations
✓ Affinity Purified ✓ 1 Citation
RRID: AB_2846785 — registered in the Antibody Registry for unambiguous reagent identification across publications.
This antibody has been independently cited in 1 peer-reviewed publication. Verify records via the Antibody Registry (AB_2846785) or PubMed.
Storage & Handling
Store at -20 °C. Stable for 12 months from date of receipt.
This antibody has been validated for ELISA, IF/ICC, IHC, WB. Recommended dilutions: WB 1:3000-1:10000, IHC 1:50-1:200 IF/ICC: 1:200. Always titrate for your specific cell/tissue type, fixation method, and detection system.
Starting dilutions: WB 1:3000-1:10000, IHC 1:50-1:200 IF/ICC: 1:200. Run a 2-fold dilution series (3–4 concentrations) using positive-control samples to identify the optimal working dilution for your assay. Detection chemistry, antigen abundance, and sample preparation all influence optimal antibody concentration.
Confirmed reactivity: Human,Mouse,Rat,Pig,Zebrafish,Bovine,Sheep,Rabbit,Goat,Guinea pig,Dog,Monkey,Hamster,Chicken,Fish. Additional predicted species may be listed in the datasheet based on sequence homology analysis. Always empirically validate in your specific organism and tissue type before a full experimental commitment.
Full-length Actin protein of human. This monoclonal Mouse antibody was generated by peptide immunisation and subsequently affinity-purified. The immunogen sequence defines the recognised epitope and governs cross-species reactivity patterns.
Store at -20 °C. Stable for 12 months from date of receipt. Aliquot upon receipt into single-use volumes to prevent repeated freeze-thaw cycles, which progressively degrade antibody performance. Store working dilutions at 4 °C for short-term use only (≤1 week). Do not dilute the stock solution before long-term storage.
This antibody is validated under denaturing conditions standard for WB (SDS-PAGE). For non-denaturing applications (IP, Co-IP), native epitope accessibility may differ — optimize conditions and include appropriate IgG isotype controls. For IHC and IF/ICC, test antigen retrieval conditions empirically as fixation can mask the target epitope.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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