AIF1/IBA1 Antibody

SKU:BHA12907888
Research Validated
Overview
Click light‑blue chips for details
Anti-AIF1/IBA1 antibody (Rabbit, polyclonal, IgG, unconjugated). Validated for Immunofluorescence, Immunohistochemistry, Western Blot. Reacts with Human, Mouse, Rat. Suitable for routine research immunoassays. Suitable for specific detection of the target in research immunoassay workflows.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Applications Immunofluorescence, Immunohistochemistry, Western Blot
Conjugate Unconjugated
Options selector
Catalog no. Size
DF6442-100UL 100 uL
DF6442-200UL 200 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 100 uL, 200 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Store at -20 °C. Stable for 12 months from date of receipt.
  • Shipping: Please contact us to confirm shipping conditions for this product.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target AIF1/IBA1
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Immunogen A synthesized peptide derived from human AIF1/IBA1, corresponding to a region within C-terminal amino acids.
Conjugate
  • Unconjugated
Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C. Stable for 12 months from date of receipt.
Catalog no. (Mfr.) DF6442
Main SKU BHA12907888
Polyclonal Antibody

Product Overview

This polyclonal antibody, raised in Rabbit, recognizes AIF1/IBA1. AIF1/IBA1 Antibody detects endogenous levels of total AIF1/IBA1. It is validated for Immunofluorescence, Immunohistochemistry, Western Blot.

Validated Applications

Applications Immunofluorescence, Immunohistochemistry, Western Blot
WB 1:500-1:2000
IHC 1:50-1:200
IF/ICC 1:100-1:500

Optimal dilutions should be determined by the end user for each application and sample type.

Immunogen

A synthesized peptide derived from human AIF1/IBA1, corresponding to a region within C-terminal amino acids.

Specifications

Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Species Reactivity Human, Mouse, Rat
Immunogen A synthesized peptide derived from human AIF1/IBA1, corresponding to a region within C-terminal amino acids.
Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
RRID AB_2838405
Storage Store at -20 °C. Stable for 12 months from date of receipt.

Reactivity Notes

Validated reactivity: Human, Mouse, Rat. Predicted reactivity (by sequence homology): Pig,Bovine,Horse,Sheep,Rabbit,Dog. Confirm predicted species with appropriate controls before critical experiments.

Safety & Handling

This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.

Related Products

Explore related antibodies and reagents for AIF1/IBA1 research from AFFINITY BIOSCIENCES LTD, or contact us for alternative formats.

Q.Which applications is this antibody validated for?
A.Validated for Immunofluorescence, Immunohistochemistry, Western Blot. Recommended dilutions: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500.
Q.What are the host, clonality, and isotype?
A.Raised in Rabbit as a polyclonal antibody, isotype IgG. The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific)..
Q.What was the immunogen?
A.A synthesized peptide derived from human AIF1/IBA1, corresponding to a region within C-terminal amino acids.
Q.Which species does it react with?
A.Validated: Human, Mouse, Rat. Predicted cross-reactivity (not validated): Porcine, Bovine, Horse, Sheep, Rabbit, Dog — run appropriate controls before use.
Q.How should I store and handle this antibody?
A.Store at -20 °C. Stable for 12 months from date of receipt. Storage buffer: see specifications. Shipped with cold-chain packaging. RUO - not for diagnostic or therapeutic use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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NAD+-Boosters Improve Mitochondria Quality Control In Parkinson's Disease Models Via Mitochondrial UPR. Advanced science (2025). [IF=15.1] PMID: 40685704

Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization. Metabolism - Clinical and Experimental (2023). [IF=10.8] PMID: 36302455

Intestinal homeostasis disrupted by Periodontitis exacerbates Alzheimer's Disease in APP/PS1 mice. Journal of neuroinflammation (2024). [IF=9.3] PMID: 39425119

Exosomes derived from bone-marrow mesenchymal stem cells alleviate cognitive decline in AD-like mice by improving BDNF-related neuropathology. Journal of neuroinflammation (2022). [IF=9.3] PMID: 35130907

The impact of repetitive neonatal procedural pain on cognitive behavioral development in male Mice: A microglial Perspective. Brain, behavior, and immunity (2026). [IF=8.8] PMID: 41241084

Red ginseng polysaccharide targets the PI3K/AKT/mTOR pathway to induce mitochondrial apoptosis in bladder cancer. International journal of biological macromolecules (2026). [IF=7.7] PMID: 41864385

17β-trenbolone increases circulating myeloid-derived MMP8 in CSDS-induced mice and drives depressive tendencies to social threat. Environmental research (2025). [IF=7.7] PMID: 40897223

Efficacy and safety of intrathecal human umbilical cord-derived mesenchymal stromal cell therapy for bone cancer pain: a preclinical study. Stem cell research & therapy (2025). [IF=7.5] PMID: 41276847

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