Anti-Human PEAR1 Recombinant Antibody (SV001)

What it means: Target names the protein this antibody detects. Here the target is PEAR1 (platelet endothelial aggregation receptor 1), a single-pass transmembrane receptor whose large extracellular part carries one EMI domain and nine EGF-like repeats.

Why it matters:

• PEAR1 becomes tyrosine-phosphorylated during platelet aggregation and takes part in platelet contact-induced activation, so it is studied in platelet function, thrombosis and antithrombotic target work.
• The receptor is also catalogued under the gene synonym MEGF12, so a PEAR1 listing and a MEGF12 listing describe one and the same receptor.
• Target and reactivity describe different things: PEAR1 is the protein, and human is the species whose PEAR1 the manufacturer states this antibody recognises.

How to interpret here: A target of PEAR1 means this antibody is directed at a cell-surface receptor, so the part of the receptor it binds decides which formats will work.

Tip: The manufacturer does not state the epitope region, so record the clone together with the target when comparing PEAR1 antibodies across suppliers.

What it means: Clonality describes whether the antibody is one defined binder or a mixed population. This product is Monoclonal, and it is expressed recombinantly from a cloned sequence rather than harvested from a hybridoma culture.

Why it matters:

• A monoclonal binds a single epitope, which gives tighter specificity and far less lot-to-lot drift than a polyclonal pool.
• Recombinant expression means the sequence is fixed, so the same clone can be re-made indefinitely without the animal-to-animal variation of serum-derived reagents.
• The trade-off is that a single epitope can be lost to fixation or denaturation, so a format that works for one monoclonal may fail for another against the same target.

How to interpret here: Monoclonal means you can expect consistent behaviour between lots, but the antibody stands or falls on whether its one epitope survives your sample preparation.

Tip: When a monoclonal gives no signal, suspect the epitope before the antibody.

What it means: Host is the species whose immunoglobulin framework the antibody is built on. This antibody is on a Human framework, which also determines the secondary reagent your assay needs.

Why it matters:

• Unless you label the antibody yourself, detection uses a labelled anti-human IgG secondary matched to your readout, not the anti-rabbit or anti-mouse reagents most primary antibodies use.
• A human framework is the awkward case for human samples: an anti-human IgG secondary will also bind the immunoglobulin already present in serum, plasma or tissue, which raises background.
• In a multiplex panel, a human-framework primary cannot share a secondary with a mouse or rabbit primary, so plan the panel around it.

How to interpret here: A Human host means standard anti-human IgG detection applies, and that endogenous immunoglobulin in human material is the first background source to control for.

Tip: For human samples, consider a directly labelled format or a Fab-specific secondary rather than a whole-molecule anti-human IgG.

What it means: Isotype is the immunoglobulin class, subclass and light chain of the antibody. This product is IgG4, kappa.

Why it matters:

• IgG4 is the human subclass with the weakest effector function: it fixes complement poorly and engages activating Fc receptors weakly, which is why it is chosen when binding is wanted without cell killing.
• That makes the subclass relevant to any functional readout, because an IgG4 is not interchangeable with an IgG1 of the same specificity if the assay depends on Fc engagement.
• IgG4 binds both Protein A and Protein G, which is consistent with the Protein A/G purification used here, and the kappa light chain allows anti-kappa detection reagents.

How to interpret here: IgG4, kappa means standard Protein A/G handling and anti-human IgG detection apply, while effector-function-dependent assays need a different subclass.

Tip: Match your isotype control to IgG4 rather than to generic human IgG.

What it means: The clone number identifies the single binder this antibody is made from. Here the clone is SV001, and because the product is recombinant, that identifier corresponds to a fixed coding sequence rather than a particular cell bank.

Why it matters:

• The clone, not the catalogue number, is what makes results comparable: the same clone number identifies the same binder wherever it appears, while two anti-PEAR1 monoclonals with different clone numbers may behave quite differently.
• Published results are reproducible only if the clone matches, so the clone belongs in your methods section alongside the supplier and lot.
• Because the clone is a fixed sequence, the binder itself does not change between lots, but the vial concentration can, so re-check your working dilution on a new lot.

How to interpret here: Clone SV001 is the identifier to quote in your methods; the manufacturer does not state which epitope it binds.

Tip: Quote the clone, the supplier and the lot in your methods section.

What it means: Reactivity lists the species whose version of the target the manufacturer states the antibody detects. Here reactivity is Human.

Why it matters:

• The stated species is the only one characterised; behaviour against mouse, rat or other PEAR1 orthologues is not described in either direction.
• PEAR1 orthologues differ in sequence, and the manufacturer does not state the epitope, so cross-species performance cannot be predicted from the data provided.
• The word human covers two different things for this product: the framework the antibody is built on, and the species whose PEAR1 it binds.

How to interpret here: Reactivity of Human means human material is the stated scope, and any other species needs in-house confirmation first.

Tip: If you need a non-human orthologue, ask us before ordering rather than testing this clone speculatively.

What it means: Applications lists the assay formats this antibody is intended for. Here that is ELISA, Flow Cytometry, Functional Assay.

Why it matters:

• ELISA is listed as an application in its own right and is the only one with a dilution range (1:2,000–1:20,000); Flow Cytometry and Functional Assay are listed under bioactivity with no protocol or titre attached.
• No validation data or per-application protocols accompany the listings, so each format still has to be optimised locally.
• Formats the manufacturer does not list, such as Western blot or immunohistochemistry, are uncharacterised for this clone rather than ruled out.

How to interpret here: Treat ELISA, Flow Cytometry, Functional Assay as the set the manufacturer supports, and plan a titration for whichever of them you run.

Tip: Because the epitope region is undefined, verify surface staining on intact cells before committing a flow panel to this clone.