Fluorescence micrograph of BeaverBeads Magrose COOH agarose microspheres, showing the uniform spherical morphology of the 30-150 um size range. Image published by BEAVER Biomedical Engineering.
BeaverBeads Magrose COOH Carboxyl Magnetic Agarose Beads
BeaverBeads Magrose COOH are superparamagnetic agarose beads (30-150 um) carrying approximately 50 umol/mL of surface carboxyl groups. EDC/NHS activation couples antibodies, proteins, peptides and amine-modified oligonucleotides covalently to the surface, turning the beads into a custom affinity support for immunoprecipitation and protein purification.
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Overview
What is BeaverBeads Magrose COOH Carboxyl Magnetic Agarose Beads?
Product Overview
BeaverBeads™ Magrose COOH are superparamagnetic microspheres built on an agarose shell around an Fe3O4 core, presenting a high density of surface carboxyl groups. The porous agarose matrix gives a large accessible surface without the non-specific adsorption typical of bare polymer surfaces, and the superparamagnetic core allows the beads to be collected in seconds on a magnetic separator and fully redispersed when the field is removed.
Carboxyl groups are the most widely used handle for covalent immobilisation. Activated with EDC and NHS, they form stable amide bonds with any ligand carrying a primary amine — antibodies, proteins, peptides, amine-modified oligonucleotides and small molecules. Once coupled, the beads become a custom affinity support for the target of your choice.
How it works
The published coupling route washes the beads into MES buffer at pH 5.0, activates the surface carboxyls with fresh EDC and NHS for 30 minutes at 25 °C, then couples 50–200 µg of ligand at pH 8.0 for 2 hours at 25 °C (or 1 hour at 25 °C followed by overnight incubation at 4 °C). Residual activated esters are quenched and the conjugate is stored in preservative buffer at 4 °C. The full procedure is in the user manual linked under Documents.
Applications
Immunoprecipitation and co-immunoprecipitation with a covalently coupled capture antibody; affinity purification of proteins, peptides and oligonucleotides; chemiluminescent immunoassay development; cell sorting; phage display screening; and construction of custom affinity supports for drug-molecule capture.
Compatibility notes
Magrose COOH is the large-particle, agarose-matrix member of the BeaverBeads COOH family. If your assay needs a monodisperse sub-micron or micron polymer bead instead — for example for automated liquid handling or flow-based detection — the Mag COOH series (300 nm, 1 µm, 2 µm, 5 µm) covers that range. Beads are compatible with manual magnetic separators and with automated magnetic-rod and plate-based workstations.
Handling and safety
Do not freeze, dry out or centrifuge these beads — all three cause irreversible aggregation and permanent loss of surface activity. Vortex or sonicate briefly to a uniform suspension before every use. A magnetic separator is required. Keep the product away from metal objects and electronic devices; superparamagnetic material and magnetic separators should be kept well clear of implanted electronic devices such as cardiac pacemakers.
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Specifications are those published by the manufacturer, BEAVER Biomedical Engineering Co., Ltd.
Details
Specifications
Applications
Applications & how to use
Immunoprecipitation (IP)
Enriches a target protein and its interacting partners from lysate using an antibody coupled to beads. Used for interaction studies and downstream WB or MS.
Protein Purification
Separating a target protein from a complex mixture while keeping it folded and active, using chromatography, spin columns, selective binding or buffer exchange. Recovery and retained activity matter more than purity alone, so an activity assay should accompany the gel at each step.
Bioconjugation
Covalent attachment of a biological ligand — antibody, protein, peptide or oligonucleotide — to a surface or another molecule. The chemistry chosen determines orientation and retained activity, so coupling through a residue distant from the binding site usually matters more than raw coupling efficiency.
Chemiluminescence Immunoassay (CLIA)
Immunoassay read by light emitted from an enzymatic or direct chemiluminescent label, giving wider dynamic range and lower detection limits than colorimetric ELISA. Magnetic microspheres are the usual solid phase, so bead uniformity and low non-specific binding translate directly into assay sensitivity and reproducibility.
Cell Separation
Isolation of a defined cell population from a mixed suspension, most often by antibody-coated magnetic beads in positive or negative selection format. Negative selection leaves the target untouched by antibody, which is preferred when downstream function or receptor availability could be affected by bead binding.
Evidence
Validation & QC
Validation data for this product isn’t published yet.
Need specific QC or validation results — titer, purity, endotoxin, or assay data — for your application? Our scientific team can share what is available for this product and lot on request.
Request validation dataManufacturing
How it's made & quality control
Supplier quality control
Magnetic and functionalized beads are QC-tested for size uniformity, binding capacity and lot-to-lot consistency for reproducible separation and purification.
Product sheets provide bead size, surface chemistry, binding capacity and recommended protocols.
Certificate of Analysis provided per lot with binding-capacity data.
Supplier-level quality statement. For lot-specific results, request the Certificate of Analysis for your batch.
Resources
Documents & downloads
Questions
Frequently asked questions
What can I couple to these beads?
Anything carrying an accessible primary amine. In practice that means antibodies, recombinant proteins, peptides, amine-modified oligonucleotides and amine-bearing small molecules. Coupling uses standard EDC/NHS carbodiimide chemistry; no proprietary reagent is needed.
How much ligand should I use?
The manufacturer's protocol adds 50-200 ug of ligand per 100 uL of bead suspension. The optimum inside that range depends on your ligand and has to be found empirically - run a small titration before committing material.
Why agarose rather than a polymer bead?
The agarose shell is porous and hydrophilic. That gives more accessible surface per bead and markedly lower non-specific protein adsorption than a bare polymer surface, which matters when you are pulling a low-abundance target out of a complex lysate. The trade-off is a larger, less monodisperse particle (30-150 um) that settles faster.
Can I centrifuge the beads instead of using a magnet?
No. Centrifugation, freezing and drying all cause irreversible aggregation. Use a magnetic separator for every separation step.
What is the difference between Magrose COOH and Mag COOH?
Magrose COOH is agarose-shelled, 30-150 um, quoted at ~50 umol/mL of carboxyl. Mag COOH is polymer-shelled and monodisperse at 300 nm, 1 um, 2 um or 5 um, with carboxyl quoted per gram. Choose Magrose for capacity and low background in manual workflows; choose Mag for small, uniform particles in automated or flow-based assays.
Answered by our team
Questions from researchers
No researcher questions have been answered for this product yet. Ask our technical team and we'll get back to you — approved answers are published here.
Ask our technical team
Reviewed by a scientist — typically answered within one business day.
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Codes apply at checkoutIn the literature
Research using this product
Every paper below mentions this product's catalogue number (or product name) in its full text — a text match, not a verified citation. Each entry shows what was measured, the sample type where the paper states it, and the sentence the catalogue number appears in, and a link to the figure showing that data — so you can confirm the use case before citing.
Auto-discovered from Europe PMC by manufacturer number / product name / keyword plus supplier. A match may reference a related product; confirm relevance before citing.
Customization
Customization & add-ons
BEAVER manufactures magnetic beads at scale and supports OEM and ODM supply. The following are available through BioHippo - contact support@biohippo.com for scope and pricing.
- Bulk volumes: beyond the catalogue pack sizes, including litre-scale production.
- Custom functionalisation: alternative surface groups (NH2, NHS, OH) and custom carboxyl densities.
- Pre-coupled beads: your antibody or protein covalently immobilised by the manufacturer.
- Custom particle sizes: outside the standard 30-150 um agarose range.
- OEM / ODM supply: for diagnostic kit and instrument manufacturers.
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