| Field | Specification |
|---|---|
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Full-length beta-Actin protein of human. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein. This is a monoclonal Mouse anti-beta-Actin antibody (Clone F929), supplied as unconjugated, validated for ELISA | IF/ICC | IHC | WB.
Validated Applications & Dilution Guide
| Application | Recommended Dilution |
|---|---|
| WB | 1:10000-1:320000 |
| IHC | 1:50-1:200 IF/ICC: 1:200 |
Antibody Specifications
| Target Protein | beta-Actin |
|---|---|
| Host Species | Mouse |
| Clonality | Monoclonal (Clone F929) |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat, Pig, Zebrafish, Bovine, Sheep, Rabbit, Goat, Guinea pig, Dog, Monkey, Hamster, Duck, Chicken, Fish |
| Purification Method | Affinity Purified |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Stable for 24 months from date of receipt. |
| Catalog No. | T0022 |
Immunogen / Antigen
Full-length beta-Actin protein of human.
Validation & Citations
✓ Affinity Purified ✓ 134 Citations
RRID: AB_2839417 — registered in the Antibody Registry for unambiguous reagent identification across publications.
This antibody has been independently cited in 134 peer-reviewed publications. Verify records via the Antibody Registry (AB_2839417) or PubMed.
Storage & Handling
Store at -20 °C. Stable for 12 months from date of receipt.
This antibody has been validated for ELISA, IF/ICC, IHC, WB. Recommended dilutions: WB 1:10000-1:320000, IHC 1:50-1:200 IF/ICC: 1:200. Always titrate for your specific cell/tissue type, fixation method, and detection system.
Starting dilutions: WB 1:10000-1:320000, IHC 1:50-1:200 IF/ICC: 1:200. Run a 2-fold dilution series (3–4 concentrations) using positive-control samples to identify the optimal working dilution for your assay. Detection chemistry, antigen abundance, and sample preparation all influence optimal antibody concentration.
Confirmed reactivity: Human,Mouse,Rat,Pig,Zebrafish,Bovine,Sheep,Rabbit,Goat,Guinea pig,Dog,Monkey,Hamster,Duck,Chicken,Fish. Additional predicted species may be listed in the datasheet based on sequence homology analysis. Always empirically validate in your specific organism and tissue type before a full experimental commitment.
Full-length beta-Actin protein of human. This monoclonal Mouse antibody was generated by peptide immunisation and subsequently affinity-purified. The immunogen sequence defines the recognised epitope and governs cross-species reactivity patterns.
Store at -20 °C. Stable for 12 months from date of receipt. Aliquot upon receipt into single-use volumes to prevent repeated freeze-thaw cycles, which progressively degrade antibody performance. Store working dilutions at 4 °C for short-term use only (≤1 week). Do not dilute the stock solution before long-term storage.
This antibody is validated under denaturing conditions standard for WB (SDS-PAGE). For non-denaturing applications (IP, Co-IP), native epitope accessibility may differ — optimize conditions and include appropriate IgG isotype controls. For IHC and IF/ICC, test antigen retrieval conditions empirically as fixation can mask the target epitope.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Elucidation of Spatial Cooperativity in Chemo-Immunotherapy by a Sequential Dual-pH-Responsive Drug Delivery System. Advanced materials (Deerfield Beach, Fla.) (2024) [IF=27.4] PMID: 38602707
Efficient Therapy of Inflammatory Bowel Disease (IBD) with Highly Specific and Durable Targeted Ta2C Modified with Chondroitin Sulfate (TACS). Advanced Materials (2023) [IF=27.4] PMID: 37224059
Positive feedback loop PU.1-IL9 in Th9 promotes rheumatoid arthritis development. Annals of the rheumatic diseases (2024) [IF=20.3] PMID: 39164066
TRβ activation confers AT2-to-AT1 cell differentiation and anti-fibrosis during lung repair via KLF2 and CEBPA. Nature communications (2024) [IF=16.6] PMID: 39375377
Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication. NUCLEIC ACIDS RESEARCH (2019) [IF=16.6] PMID: 30462330
The missense mutation Y65C in PQBP1 causes microcephaly and cognitive deficits through a combination of partial loss-of-function and gain-of-function effects. Nature communications (2026) [IF=16.6] PMID: 41507200
A covalent peptide-based lysosome-targeting protein degradation platform for cancer immunotherapy. Nature communications (2025) [IF=16.6] PMID: 39910101
Histone lactylation regulates DOCK4 to control heat nociception and supports Dynein-mediated Nav1.7 trafficking. Nature communications (2025) [IF=16.6] PMID: 40759894
A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages. Nature Communications (2022) [IF=16.6] PMID: 36333306
Keratinocyte-specific H4K12 lactylation drives a non-canonical IL-17-dependent signaling in psoriasis progression in mice. Nature communications (2025) [IF=16.6] PMID: 41027887
