| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FAK, corresponding to a region within the internal amino acids. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes FAK. FAK Antibody detects endogenous levels of total FAK. It is validated for Immunofluorescence, Immunohistochemistry, Western Blot.
Validated Applications
| Applications | Immunofluorescence, Immunohistochemistry, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:50-1:200 |
| IF/ICC | 1:100-1:500 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthesized peptide derived from human FAK, corresponding to a region within the internal amino acids.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | A synthesized peptide derived from human FAK, corresponding to a region within the internal amino acids. |
| Purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). |
| Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RRID | AB_2835232 |
| Storage | Store at -20 °C. Stable for 12 months from date of receipt. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. Predicted reactivity (by sequence homology): Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus. Confirm predicted species with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for FAK research from AFFINITY BIOSCIENCES LTD, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dysregulated ITGA3/FAK/YAP axis mediates impaired alveolar type II epithelial cells function in COPD. Journal of advanced research (2025). [IF=11.4] PMID: 41270956
Hydroxylsafflower yellow A modulates tumor vascularization in liver cancer through intercellular communication and extracellular matrix-receptor interactions. Phytomedicine (2025). [IF=6.7] PMID: 41197344
Anti-proliferation and anti-migration effects of Yishen Tongbi decoction in experimental rheumatoid arthritis by suppressing SLC3A2/integrin β3 signaling pathways. Phytomedicine (2023). [IF=6.7] PMID: 36990010
Catalpol ameliorates liver fibrosis via inhibiting aerobic glycolysis by EphA2/FAK/Src signaling pathway. Phytomedicine (2024). [IF=6.7] PMID: 39321687
pH-responsive biomimetic zeolitic imidazolate framework-based nanoparticles for co-delivery of cetuximab and siRNA in synergistic therapy of laryngeal squamous cell carcinoma. Journal of pharmaceutical analysis (2025). [IF=6.1] PMID: 40735032
Analysis of Expression of the ANG1, CaSR and FAK Proteins in Uterine Fibroids. International journal of molecular sciences (2024). [IF=5.6] PMID: 39000274
LRRC8A/TRIM21 axis exacerbates myocardial ischemia/reperfusion injury by blocking FAK/NF-κB pathway. Biochemical pharmacology (2025). [IF=5.3] PMID: 40619028
Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro. Journal of Ethnopharmacology (2019). [IF=4.8] PMID: 30445110
Phellinus linteus activates Treg cells via FAK to promote M2 macrophage polarization in hepatocellular carcinoma. Cancer immunology, immunotherapy (2024). [IF=4.6] PMID: 38240856
FOXF1 inhibits invasion and metastasis of lung adenocarcinoma cells and enhances anti-tumor immunity via MFAP4/FAK signal axis. Scientific reports (2024). [IF=3.8] PMID: 39271782
