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Product Overview
HepG2, a well-characterized human hepatocellular carcinoma line, was taken from liver tissue of a 15-year-old male donor whose hepatocellular carcinoma was differentiated. Epithelial morphology and the retention of many liver-specific functions have pushed the line to the front rank of in vitro models for hepatic metabolism, gene regulation, drug discovery, toxicology, viral infection, and recombinant protein expression. Plasma proteins synthesized and secreted by HepG2 cells include albumin, transferrin, α1-antitrypsin, α2-macroglobulin, and plasminogen, and the cells answer a variety of hormonal and metabolic stimuli.
Robust growth characteristics plus adaptability to large-scale culture systems have widened their use further across biomedical and pharmaceutical research. GenJet™ DNA In Vitro Transfection Reagent for HepG2 Cells comes tuned to the line, delivering plasmid DNA into HepG2 cells efficiently.
Specifications
| Transfection Type | DNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | Yes — Pre-Optimized for Specific Cell Type |
| Optimized Cell Line | HepG2 |
| Storage | Store at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months. |
Kit Contents
One milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates, depending on the culture format and amount of DNA used.
Performance Data
Applications
This DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
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