| Field | Specification |
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Product Overview
MCF-7, a human breast adenocarcinoma cell line in wide circulation, is the target of the pre-optimized GenMute™ siRNA Transfection Reagent for MCF-7 Cells, which moves siRNA into these cells efficiently. Isolated in 1970 from a Caucasian woman aged 69, the line holds on to much of the character of differentiated mammary epithelium — hormone responsiveness and estrogen receptor (ER) expression among those traits. Investigators turn to it for estrogen signaling, breast cancer biology, proliferation, apoptosis, and how cells answer therapy.
Oncogenic pathways such as Wnt7B are expressed here too, and the cells react to cytokines like tumor necrosis factor-α (TNF-α). Low cytotoxicity alongside high transfection efficiency makes dependable gene silencing and in vitro functional studies possible.
Specifications
| Transfection Type | siRNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | Yes — Pre-Optimized for Specific Cell Type |
| Optimized Cell Line | MCF-7 |
| Storage | Store at 4 °C. If stored properly, the product is stable for 24 months or longer |
Optimal Transfection Conditions
| Cell Culture Medium | DMEM with 4.5 g/L glucose, 10% FBS |
|---|---|
| Cell Confluency on the day of transfection | ~50% |
| Optimal siRNA Concentration | 10 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 90% |
Kit Contents
- GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate
- GenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make a 40 mL working solution
Applications
This siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Du J, Liao W, Wang H, et al.. MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration. Signal Transduct Target Ther (2023) 8(1), 351-351.. 10.1038/s41392-023-01575-5
Xu T, Ma Q, Li Y, et al.. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduct Target Ther (2022) 7(1), 354-354.. 10.1038/s41392-022-01182-w
Sladitschek-Martens H, Guarnieri A, Brumana G, et al.. YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING. Nature (2022) 607(7920), 790-798.. 10.1038/s41586-022-04924-6
Meunier E, Dick M, Dreier R, et al.. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature (2014) 509(7500), 366-70.. 10.1038/nature13157
