| Field | Specification |
|---|---|
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| Catalog no. (Mfr.) | |
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Product Overview
Neuro-2A (N2A) cells — a mouse neuroblastoma line serving broadly as an in vitro model for neuronal differentiation, neurodevelopment, neurotoxicity, and signal transduction studies — are what the pre-optimized GenMute™ siRNA Transfection Reagent for Neuro-2A Cells addresses, delivering siRNA into them efficiently. Transfection efficiency across N2A cultures can vary when conventional reagents are used, the more so once the cells take on a neuron-like differentiated state. This formulation sets out to raise cellular uptake and endosomal release of siRNA without compromising viability or normal cellular morphology.
Optimal gene knockdown depends on following the transfection conditions recommended for Neuro-2A cells: cell density, siRNA amount, and incubation parameters.
Specifications
| Transfection Type | siRNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | Yes — Pre-Optimized for Specific Cell Type |
| Optimized Cell Line | Neuro-2A |
| Storage | Store at 4 °C. If stored properly, the product is stable for 24 months or longer |
Optimal Transfection Conditions
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
|---|---|
| Confluency of the cell on the day of transfection | ~50% |
| Optimal siRNA concentration | 10 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 90% |
Kit Contents
- GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate
- GenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Applications
This siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
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Xu T, Ma Q, Li Y, et al.. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduct Target Ther (2022) 7(1), 354-354.. 10.1038/s41392-022-01182-w
Sladitschek-Martens H, Guarnieri A, Brumana G, et al.. YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING. Nature (2022) 607(7920), 790-798.. 10.1038/s41586-022-04924-6
Meunier E, Dick M, Dreier R, et al.. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature (2014) 509(7500), 366-70.. 10.1038/nature13157
