| Field | Specification |
|---|---|
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
Efficient siRNA delivery into RAW 264.7 cells is the purpose GenMute™ siRNA Transfection Reagent for RAW 264.7 Cells was optimized around. The line itself is macrophage-like and murine, obtained from BALB/c mice and transformed using Abelson murine leukemia virus. Inflammation, macrophage biology, host–pathogen interactions, and signal transduction are common subjects of study in it.
Once transfected, the cells still suit routine immunocytochemistry protocols aimed at protein expression and protein–protein interactions.
Specifications
| Transfection Type | siRNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | Yes — Pre-Optimized for Specific Cell Type |
| Optimized Cell Line | RAW 264.7 |
| Storage | Store at 4 °C. If stored properly, the product is stable for 24 months or longer. |
Optimal Transfection Conditions
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
|---|---|
| Confluency of cell on the day of transfection | ~70% |
| Optimal siRNA concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | 90% |
Kit Contents
- GenMute™ Reagent (1.0 mL): Sufficient for approximately 833 transfections based on delivery of 15 pmol siRNA or miRNA mimics per well in a 24-well plate
- GenMute™ Transfection Buffer (5×, 8.0 mL): Optimized for maximal transfection efficiency; yields 40 mL of 1× working solution
Applications
This siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Du J, Liao W, Wang H, et al.. MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration. Signal Transduct Target Ther (2023) 8(1), 351-351.. 10.1038/s41392-023-01575-5
Xu T, Ma Q, Li Y, et al.. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduct Target Ther (2022) 7(1), 354-354.. 10.1038/s41392-022-01182-w
Sladitschek-Martens H, Guarnieri A, Brumana G, et al.. YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING. Nature (2022) 607(7920), 790-798.. 10.1038/s41586-022-04924-6
Meunier E, Dick M, Dreier R, et al.. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature (2014) 509(7500), 366-70.. 10.1038/nature13157
