| Field | Specification |
|---|---|
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U2OS cells receive siRNA efficiently with GenMute™ siRNA Transfection Reagent for U2OS Cells, a formulation optimized for this line. Established in 1964, U2OS came originally from the tibial bone of a female patient aged 15 and is of human osteosarcoma origin. Its adherent, epithelial-like morphology accompanies broad use in gene function work, DNA damage response, cell cycle regulation, and cancer biology.
Receptors for insulin-like growth factor I (IGF-I) and II (IGF-II) are expressed by U2OS cells, and the line is well characterized for growing and transfecting consistently in vitro.
Specifications
| Transfection Type | siRNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | Yes — Pre-Optimized for Specific Cell Type |
| Optimized Cell Line | U2OS |
| Storage | Store at 4 °C. If stored properly, the product is stable for 24 months or longer |
Optimal Transfection Conditions
| Cell Culture Medium | DMEM with 4.5 g/L glucose, 10% FBS |
|---|---|
| Cell Confluency on the day of transfection | ~50% |
| Optimal siRNA Concentration | 5 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 90% |
Kit Contents
- GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 15 pmol siRNA or miRNA mimics in a 24-well plate
- GenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make a 40 mL working solution
Applications
This siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Du J, Liao W, Wang H, et al.. MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration. Signal Transduct Target Ther (2023) 8(1), 351-351.. 10.1038/s41392-023-01575-5
Xu T, Ma Q, Li Y, et al.. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduct Target Ther (2022) 7(1), 354-354.. 10.1038/s41392-022-01182-w
Sladitschek-Martens H, Guarnieri A, Brumana G, et al.. YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING. Nature (2022) 607(7920), 790-798.. 10.1038/s41586-022-04924-6
Meunier E, Dick M, Dreier R, et al.. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature (2014) 509(7500), 366-70.. 10.1038/nature13157
