HEK293T-eGFP Stable Cell Line

What it means: This stable cell line is derived from HEK293T cells, a widely used human embryonic kidney cell line.

Why it matters:

• HEK293T cells are highly transfectable and support robust transgene expression, making them ideal for generating stable reporter cell lines.
• The SV40 large T antigen in HEK293T cells enhances episomal replication of plasmids containing the SV40 origin.

How to interpret here: HEK293T provides a well-characterized, adherent human cell background for your experiments with established culture protocols.

What it means: This cell line is of Human origin, derived from human embryonic kidney cells.

Why it matters:

• Human-origin cell lines express endogenous human proteins and post-translational modifications relevant to human disease modeling.
• Results from Human cell lines are directly translatable to human biology research contexts.

How to interpret here: This Human cell line is suitable for studies requiring human cellular context, including drug screening, protein expression, and signaling pathway analysis.

What it means: This product requires BSL-2 containment for safe handling in the laboratory.

Why it matters:

• BSL-2 is required because this cell line was generated using lentiviral transduction, which involves integration of genetic material into the host genome.
• Proper biosafety practices protect laboratory personnel and prevent environmental release of genetically modified organisms.

How to interpret here: Ensure your facility has BSL-2 certification and follow your institutional biosafety committee (IBC) guidelines before working with this cell line.

What it means: This product is classified as a Stable Cell Line, meaning the transgene is stably integrated into the host cell genome.

Why it matters:

• Stable cell lines maintain consistent transgene expression across passages without the need for repeated transfection.
• Antibiotic selection pressure ensures that only cells retaining the integrated construct are maintained in culture.

How to interpret here: This Stable Cell Line provides a reliable, ready-to-use research tool with reproducible transgene expression for your experiments.

What it means: Each lot of this cell line has been tested for mycoplasma contamination and confirmed Negative.

Why it matters:

• Mycoplasma contamination can alter cell behavior, gene expression, and experimental reproducibility without visible signs of infection.
• Starting with a mycoplasma-negative cell line ensures reliable baseline data for your research.

How to interpret here: This product meets quality standards for mycoplasma-free status. Routine testing during culture is still recommended as best practice.

What it means: This product should be stored in Liquid nitrogen vapor phase for long-term preservation.

Why it matters:

• Liquid nitrogen vapor phase storage maintains cell viability and transgene expression stability over extended periods.
• Storing at higher temperatures (−20°C or −80°C) can reduce post-thaw viability and transgene expression levels.

How to interpret here: Upon receipt, transfer the cryopreserved vial immediately to Liquid nitrogen vapor phase storage. Thaw only when ready to culture.

What it means: This cell line is supplied in Cryopreserved format, ready for thawing and culture.

Why it matters:

• Cryopreserved cells can be stored long-term in liquid nitrogen and recovered when needed, providing flexible experimental scheduling.
• Each vial contains approximately 3 × 10⁶ viable cells, sufficient to establish a culture from a single thaw.

How to interpret here: Thaw the cryopreserved vial rapidly at 37°C and transfer to pre-warmed complete medium. Cells typically attach within 12–24 hours.