| Field | Specification |
|---|---|
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Novel fluorinated cationic lipids form the basis of LipoJet™ Transfection Kit, which is built on SignaGen's innovative, proprietary lipid-conjugation technology. SignaGen presents the kit as the most powerful gene delivery tool available and at the same time a very gentle one, holding significant advantages over competing lipid transfection reagents on the market, and as the more cost-effective option next to leading products there, always giving higher transfection efficiency with less cytotoxicity. Its applications span plasmid DNA, siRNA, or the two together, across most mammalian cell types.
Specifications
| Transfection Type | DNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | No — General-Purpose Formulation |
| Storage | Store at 4 °C for LipoJet™ Reagent and RT for LipoJet™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer. |
Kit Contents
- LipoJet™ Reagent, 1.0 ml sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate)
- LipoJet™ Transfection Buffer (5x), 8.0 mL to make 40 mL of working solution (1x )
Features
- Versatile: DNA transfection, siRNA transfection and combined DNA/siRNA co-transfection
- Powerful: outstanding efficiency in adherent cells
- Extremely low toxicity: better cell viability following transfection
- Economical: more transfections per unit of reagent
- Easy to use: single-tube reaction, compatible with serum/antibiotics
- Suited to high-throughput work
Performance Data
Transfection Efficiency by Cell Line
| Cell Line | DNA Transfection Efficiency | siRNA Transfection Efficiency |
|---|---|---|
| 293, 293T | 85% | – |
| 3T3, NIH 3T3 | 65% | 90% at 20 nM |
| 3T3-L1 | 30% | – |
| A549 | 80% | 90% at 10 nM |
| B16-F10 | 75% | – |
| BNLCL.2 | 55% | – |
| C2C12 | 80% | 90% at 10 nM |
| C3H/10T1/2 | 60% | – |
| Caco-2 | 20% | 80 % at 20 nM |
| Rat primary cardiomyocytes | 10% | – |
| CHO | 80% | – |
| COS1 | 70% | – |
| COS7 | 75% | – |
| Human bladder carcinomal cell | 80% | – |
| mES | 50% | – |
| iMEF | 55% | – |
| HBEC | 45% | – |
| HCT116 | 75% | – |
| Hela | 80% | – |
| HepG2 | 60% | – |
| HT-29 | 45% | – |
| HuH-7 | 45% | – |
| LNCaP | 45% | – |
| MC3T3-E1 | 50% | – |
| MCF10A | 40% | – |
| MCF7 | 50% | – |
| MDA-MB-231 | 50% | – |
| MDCK | 20% | – |
| MEF | 50% | 85% at 30 nM |
| Human primary melanocytes | 50% | 85% at 30 nM |
| Human primary melanoma | 50% | – |
| Human immortalized microglial | 50% | – |
| mIMCD-3 | 50% | – |
| MRC-5 | 50% | – |
| N2A | 68% | – |
| PC3 | 70% | – |
| RAW 264.7 | 45% | – |
| RPE | 30% | – |
| SH-SY-5Y | 75% | 90% at 25 nM |
| SK-OV-3 | 70% | – |
| U-2OS | 80% | – |
| VERO | 40% | – |
| hESCs | 70% | 90% at 40 nM |
Applications
This DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
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