| Field | Specification |
|---|---|
| Alternative names | LV-EF1a-Null-Puro | LV-EF1α-Null-Puro | LV-EF1α-Null-IRES-Puro | EF1α-Null-Puro Lentivirus | EF1A-Null-Puro Lentivirus |
| Applications | |
| Purity | |
| Titer | |
| Promoter | |
| Expression regulation | |
| Selection marker | Puromycin |
| Biosafety level | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Vector Overview
LV-EF1a-Null-Puro is a pre-packaged recombinant lentiviral vector that carries no transgene under the human elongation factor 1-alpha (EF1a) promoter, serving as a negative control. The EF1a promoter is a strong, ubiquitous mammalian promoter derived from the human EF1A gene. It provides sustained, high-level expression across a wide range of cell types, including primary cells and stem cells, and is less susceptible to transgene silencing than viral promoters such as CMV. The puromycin resistance cassette allows efficient antibiotic selection of successfully transduced cells, enabling rapid generation of stable, enriched cell populations. This design makes LV-EF1a-Null-Puro an essential matched control for experiments involving lentiviral transduction under the same promoter, ensuring that observed phenotypic effects are attributable to the transgene of interest rather than to the viral backbone, promoter activity, or the transduction process itself. Lentiviral delivery ensures efficient, stable integration into the host genome of both dividing and non-dividing mammalian cells. LV-EF1a-Null-Puro is supplied in a ready-to-use format, eliminating the need for vector construction, packaging, and titration.
Technical Details
| Catalog No. | SL100262 |
|---|---|
| Promoter | EF1a |
| Expression Regulation | Constitutive |
| Selection Marker | Puromycin |
| Titer | >1E+9 TU/mL |
| Storage Buffer | PBS |
| Purity / Grade | In vivo grade and OK for both in vitro tissue culture infection and in vivo injection * Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm |
| Biosafety Level | BSL-2 |
| Sizes | 25 uL; 25 uL x 2 |
Applications
- Lentiviral Transduction: stable delivery of the cassette into dividing and non-dividing cells for long-term expression.
Biosafety
LV-EF1a-Null-Puro is a pre-packaged lentiviral vector classified as BSL-2. Handle in a certified biosafety cabinet under institutional BSL-2 practices, decontaminate all contact surfaces and waste, and follow local regulations for viral vector work.
Safety & Handling
Store at -80°C; avoid repeated freeze-thaw cycles. Ships on dry ice. Supplied in PBS. For research use only; not for diagnostic or therapeutic use.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
