| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nicotinic Acetylcholine Receptor alpha 7, corresponding to a region within N-terminal amino acids. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Nicotinic Acetylcholine Receptor alpha 7. Nicotinic Acetylcholine Receptor alpha 7 Antibody detects endogenous levels of total Nicotinic Acetylcholine Receptor alpha 7. It is validated for Immunofluorescence, Immunohistochemistry, Western Blot.
Validated Applications
| Applications | Immunofluorescence, Immunohistochemistry, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
| IF/ICC | 1:100-1:500 |
| IHC | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthesized peptide derived from human Nicotinic Acetylcholine Receptor alpha 7, corresponding to a region within N-terminal amino acids.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | A synthesized peptide derived from human Nicotinic Acetylcholine Receptor alpha 7, corresponding to a region within N-terminal amino acids. |
| Purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). |
| Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RRID | AB_2846266 |
| Storage | Store at -20 °C. Stable for 12 months from date of receipt. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. Predicted reactivity (by sequence homology): Chicken. Confirm predicted species with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Nicotinic Acetylcholine Receptor alpha 7 research from AFFINITY BIOSCIENCES LTD, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Targeting α7nAChR-driven brain endothelial pyroptosis mitigates HIV-1 gp120-induced blood-brain barrier breakdown. Brain, behavior, and immunity (2026). [IF=8.8] PMID: 41651130
Gelatin nanoparticles loaded with 3-alkylpyridinium salt APS7, an analog of marine toxin, are a promising support in human lung cancer therapy. Biomedicine & pharmacotherapie (2024). [IF=6.9] PMID: 38906020
Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress. International journal of molecular sciences (2024). [IF=5.6] PMID: 38892090
α7nAChR-mediated astrocytic activation: a novel mechanism of Xiongzhi Dilong decoction in ameliorating chronic migraine. Journal of ethnopharmacology (2024). [IF=4.8] PMID: 38971346
α-Mangostin Treats Early-Stage Adjuvant-Induced Arthritis of Rat by Regulating the CAP-SIRT1 Pathway in Macrophages. Drug Design, Development and Therapy (2022). [IF=4.7] PMID: 35250263
Acupuncture alleviates inflammatory pain by regulating microglial polarization through α7nAchR-mediated autophagy. Journal of integrative medicine (2026). [IF=4.2] PMID: 41826216
SAM Alleviates Neuroinflammation by Regulating M1/M2 Polarization of Microglia Through α7nAChR/Nrf2/HO-1 Signaling Pathway. Neurochemical research (2025). [IF=3.7] PMID: 40156718
Targeting α7nAChR mitigates vascular aging and endothelial cell senescence through inhibiting oxidative stress and inflammation via α7nAChR/Nrf2/HO-1 signaling pathway. Archives of gerontology and geriatrics (2025). [IF=3.5] PMID: 41192228
Activation of α7nAChR by PNU282987 improves cognitive impairment through inhibiting oxidative stress and neuroinflammation in D-galactose induced aging via regulating α7nAChR/Nrf2/HO-1 signaling pathway. Experimental Gerontology (2023). [IF=3.3] PMID: 36898594
The prenatal hypoxic pathology associated with maternal stress predisposes to dysregulated expression of the chrna7 gene and the subsequent development of nicotine addiction in adult offspring. Neuroendocrinology (2024). [IF=3.2] PMID: 38198758
