| Field | Specification |
|---|---|
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
At the heart of PepJet™ In Vivo DNA Transfection Reagent sits a synthetic 28-amino-acid peptide, picked out originally by screening as a potent nucleic acid delivery vehicle and then refined with sequence alignment, molecular modeling, and computational analyses that took their cue from naturally occurring viral protein architectures. Chemical synthesis, structural refinement, and repeated rounds of in vitro and in vivo validation followed, all aimed at wringing the most gene transfer efficiency out of it; what resulted is a next-generation cationic peptide–based delivery system engineered for highly efficient plasmid DNA transfection in vivo. A proprietary shielding peptide is covalently built into the platform for better systemic stability and biological performance: it cuts down nonspecific interactions with serum proteins and other biological components once the material is given systemically, so PepJet™/DNA complexes form, hold together, and distribute better.
Animal studies using systemic administration have shown efficient plasmid DNA delivery and robust transgene expression across several organs — lung, liver, spleen, and kidney.
Specifications
| Transfection Type | In Vivo Transfection |
|---|---|
| Application Setting | In Vivo |
| Pre-Optimized | No — General-Purpose Formulation |
| Storage | Store at 4°C. When maintained under the recommended storage conditions, PepJet™ DNA In Vivo Transfection Reagent is stable for at least 24 months. |
Performance Data
Applications
Suitable for in vivo gene delivery applications including gene therapy research, in vivo reporter assays, and preclinical studies. Consult the product datasheet for recommended dosing and administration routes.
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Moghadam F, LeGraw R, Velazquez J, et al.. Synthetic immunomodulation with a CRISPR super-repressor in vivo. Nat Cell Biol (2020) 22(9), 1143-1154.. 10.1038/s41556-020-0563-3
Charidemou E, Noberini R, Ghirardi C, et al.. Hyperacetylated histone H4 is a source of carbon contributing to lipid synthesis. EMBO J (2024) 43(7), 1187-1213.. 10.1038/s44318-024-00053-0
Matsumiya T, Ding J, Ikegami S, et al.. Bacterial transfer-messenger RNA activates antiviral RNA sensing to induce inflammatory innate immune responses. J Immunol (2026) 215(6), .. 10.1093/jimmun/vkag149
Kuwajima Y, Nagai M, Lee C, et al.. Trans-trigeminal transport of masseter-derived neprilysin to hippocampus. Arch Oral Biol (2020) 118, 104861.. 10.1016/j.archoralbio.2020.104861
