| Field | Specification |
|---|---|
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
Viral cell-penetrating peptides (CPPs) provided the inspiration for PepMute™ siRNA Transfection Reagent, a novel siRNA delivery platform. SignaGen puts its gene silencing at exceptionally high levels — over 95% knockdown at 1 nM siRNA, across a variety of mammalian cell types. The reagent came out of SignaGen's proprietary Peptide Simulation Technology (PST): hundreds of viral CPPs were simulated computationally, then synthesized and screened for how well they delivered genes into diverse mammalian cells.
PepMute™ emerged from that work, validated as an exceptionally effective vector for condensing short (under 100 bp) single- or double-stranded nucleic acids — siRNAs, miRNA mimics, DNA oligonucleotides — and carrying them into a broad spectrum of mammalian cells.
Specifications
| Transfection Type | siRNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | No — General-Purpose Formulation |
| Storage | Store at 4 °C for PepMute™ Reagent and RT for PepMute™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer. |
Kit Contents
- PepMute™ Reagent, 1.0 mL, sufficient for ~1,333 reactions based on transfecting 0.5~5.0 pmol siRNA or miRNA mimics in a 24-well plate
- PepMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL at 5x concentrated stock solution to make 40 mL of working solution
Features
- Excellent silencing at a final siRNA concentration of 1.0 nM
- Over 95% gene silencing across a wide variety of cells
- Single-tube reaction with a simple standard protocol
- Works in the presence of serum and antibiotics
- Protocols adapted for HTS
- Cytotoxicity kept very low
- Highly affordable
Performance Data
Applications
- Transfection of siRNA, miRNA mimics, or mRNA
- Co-transfection of DNA and siRNA
- Transfection of DNA oligos
Safety & Handling
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
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Yang J, Morris A, Kamizaki K, et al.. ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1. Cell (2025) 188(10), 2569-2585.e20.. 10.1016/j.cell.2025.03.019
