PepMute™ Transfection Buffer (5x)

SKU:BHT2200060
Research Validated
Overview
Click light‑blue chips for details
PepMute™ Transfection Buffer (5x) is the 5x transfection buffer formulated for PepMute™ siRNA and DNA transfection reagents. It is used to dilute the siRNA or DNA and the transfection reagent so that the transfection complex forms efficiently. Supplied as an 8.0 mL vial of 5x stock that makes 40 mL of 1x working solution.
Transfection Type Buffer
Storage RT
Shelf Life 24 months
Options selector
Catalog no. Size
SL100575-1P0ML 1.0 mL
SL100575-4P0ML 4.0 mL
SL100575-8P0ML 8.0 mL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: 3 variants available — select size from the variant selector.
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Store at RT. If stored properly, the product is stable for 24 months or longer.
  • Shipping: Please contact us to confirm shipping conditions for this product.
  • Upon receipt: refrigerate upon receipt.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Storage RT
Catalog no. (Mfr.) SL100575
Main SKU BHT2200060

Product Overview

PepMute™ Transfection Buffer was developed for use with PepMute™ siRNA & DNA Transfection Reagent to give optimal transfection efficiency and maximum knockdown of gene expression. The buffer serves to dilute siRNA/DNA and PepMute™ Transfection Reagent so that the most efficient transfection complex forms. Supplied as a 5x concentrated stock solution, PepMute™ Transfection Buffer, 8.0 mL, makes 40 mL of working solution.

Specifications

Transfection Type Buffer
Pre-Optimized No — General-Purpose Formulation
Storage Store at RT. If stored properly, the product is stable for 24 months or longer.

Kit Contents

  • PepMute™ Transfection Buffer, 8.0 mL/vial, to make 40 mL of working solution (1x ), sufficient for 1000 transfections in 24-well plate

Applications

This transfection buffer is used to prepare transfection complexes with the matching SignaGen transfection reagent, for both DNA and siRNA delivery. Use it to dilute the nucleic acid and the reagent as described in the protocol.

Safety & Handling

For Research Use Only (RUO) — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.
Q.What is PepMute™ Transfection Buffer (5x)?
A.It is a specialized transfection buffer designed to optimize nucleic acid delivery. This buffer is used in combination with SignaGen transfection reagents to prepare transfection complexes.
Q.How should I store PepMute™ Transfection Buffer (5x)?
A.Store at RT. If stored properly, the product is stable for 24 months or longer. Follow the product datasheet for detailed storage and stability information.
Q.What is included with PepMute™ Transfection Buffer (5x)?
A.The vendor lists: PepMute™ Transfection Buffer, 8.0 mL/vial, to make 40 mL of working solution (1x ), sufficient for 1000 transfections in 24-well plate.
Q.Is this product suitable for diagnostic or therapeutic use?
A.No. This product is for Research Use Only (RUO) and is not intended for diagnostic, therapeutic, or clinical applications.
Q.How can I request pricing for this product?
A.Click "Request special pricing" on the product page or contact BioHippo support. Volume discounts may be available for bulk orders.

Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

Wu X, Wang C, Ishimwe N, et al.. MAPK14 converges on key transcriptional machinery to promote vascular smooth muscle cell degeneration in abdominal aortic aneurysm. Signal Transduct Target Ther (2026) 11(1), 17.. 10.1038/s41392-025-02540-0

Patil D, Chen C, Pickering B, et al.. m(6)A RNA methylation promotes XIST-mediated transcriptional repression. Nature (2016) 537(7620), 369-373.. 10.1038/nature19342

Zhang L, Zhang S, Yao J, et al.. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature (2015) 527(7576), 100-104.. 10.1038/nature15376

Yang J, Morris A, Kamizaki K, et al.. ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1. Cell (2025) 188(10), 2569-2585.e20.. 10.1016/j.cell.2025.03.019

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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